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Decursin from Angelica gigas Nakai Promotes Cytotoxicity and Induces Apoptosis in THP-1 cells, a Human Acute Monocytic Leukemia  

Kim, Nam-Seok (Department of Food & Biotechnology, Woosuk University)
Jeong, Seung-Il (Jeonju Agrobio-Materials Institute (JAMI))
Kim, Jong-Seok (Department of Rehabilitation studies, Woosuk university)
Oh, Mi-Jin (Korea Food Research Institute)
Oh, Chan-Ho (Department of Food & Biotechnology, Woosuk University)
Publication Information
Korean Journal of Pharmacognosy / v.47, no.3, 2016 , pp. 197-203 More about this Journal
Abstract
Decursin is a major component of the root of Angelica gigas(Umbelliferae), which has been traditionally used in Korea as a tonic and to treat anemia, hemiplegia, and women's diseases. The objective of this study is to identify the anti-cancer mechanism induced by decursin on apoptosis of human leukemia and lymphoma cells. Cytotoxicity of decursin on U937, HL-60, MOLT-4, THP-1 cells showed the significant effects. First of all, $IC_{50}$ of decursin on four cell lines was 27.1, 32.4, 17.4, $15.1{\mu}M$, respectively. So $IC_{50}$ in THP-1 cells was the smallest among 4 cell lines treated with decursin($15.1{\mu}M$). In order to understand the apoptosis-mechanism by decursin, we examined the gene expression of bcl-2(anti-apoptotic), bax(pro-apoptotic) and p53(tumor suppressor)after treating the THP-1 cells with decursin(10, 50 and $100{\mu}M$). It was found bcl-2 gene was decreased dose dependently, the expression level of bax gene of THP-1 cells treated with $100{\mu}M$ of decursin was about 3 times higher than those of control, and p53 gene was increased In the same concentration($100{\mu}M$), p53 gene was increased dose dependent manner. In protein express, bcl-2 and p53 protein showed a tendency to decrease. bax was increased about 4 fold. Therefore decursin is a useful chemotherapeutic agent against leukemia.
Keywords
Decursin; Apoptosis; Bcl family; p53; THP-1 cells;
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Times Cited By KSCI : 8  (Citation Analysis)
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