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A New Analysis of p-Coumaric Acid and Trans-cinnamic Acid in Propolis By UPLC  

Kim, Se Gun (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
Hong, In Pyo (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
Woo, Soon Ok (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
Jang, Hye Ri (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
Han, Sang Mi (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
Publication Information
Korean Journal of Pharmacognosy / v.47, no.2, 2016 , pp. 186-191 More about this Journal
Abstract
A new UPLC-DAD method for simultaneous determination of p-coumaric acid and trans-cinnamic acid in propolis was developed using Halo-C18 column and step gradient elution of MeCN and $0.1%H_3PO_4$ in 8 min. The method was validated by specificity, linearity, limit of detection, limit of quantification, precision(intra- and inter-day variability) and recovery tests. The validated method was sufficiently applicable for quantitative analysis of propolis products as well as propolis collected from different regions in Korea. The established method is simple, appropriate and efficient, and can be used for quality assessment of Korean propolis.
Keywords
Propolis; p-Coumaric acid; trans-Cinnamic acid; UPLC method;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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