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Quantitative Analysis of the Three Marker Compounds in Sanguisorbae Radix by Processing Method  

Seo, Chang-Seob (K-herb Research Group, Korea Institute of Oriental Medicine)
Kim, Jung-Hoon (K-herb Research Group, Korea Institute of Oriental Medicine)
Shin, Hyeun-Kyoo (K-herb Research Group, Korea Institute of Oriental Medicine)
Kim, Byoung-Soo (Department of Physiology, College of Korean Medicine, Daejeon University)
Publication Information
Korean Journal of Pharmacognosy / v.46, no.4, 2015 , pp. 342-351 More about this Journal
Abstract
In this study, we performed quantitative determination of the three marker compounds such as gallic acid, ellagic acid, and quercetin in the 70% ethanol extracts of non-processed Sanguisorbae Radix and processed Sanguisorbae Radix using a high-performance liquid chromatography coupled with photodiode array detector. The analytical column for separation of the three compounds was used a Gemini $C_{18}$ column ($5{\mu}m$, $4.6{\times}250mm$) by the gradient elution with distilled water and acetonitrile containing 1.0% (v/v) acetic acid as mobile phase. The flow rate and injection volume were $1.0{\mu}L/min$ and $10{\mu}L$. The concentrations of gallic acid, ellagic acid, and quercetin in non-processed Sanguisorbae Radix were 0.25, 0.26, and 0.007%, respectively, while the concentrations of gallic acid, ellagic acid, and quercetin in non-processed Sanguisorbae Radix 0.14-0.55, 0.27-2.03, and 0.001-0.007%, respectively. Among the three components, the amount of the ellagic acid was increased after processing in Sanguisorbae Radix.
Keywords
Sanguisorbae Radix; Processing Method; Gallic acid; Ellagic acid; Quercetin;
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1 Seo, C. S., Kim, J. H., Shin, H. K. and Kim, B. S. (2015) Quantitative determination of the six marker compounds in Eucommiae Cortex by processing method. Kor. J. Pharmacogn. 46: 123-132.
2 전국한의과대학 공동교재편찬위원회 편저 (2004) 본초학, 553. 영림사, 서울.
3 식품의약품안전처 (2007) 대한약전외한약(생약)규격집, 7-10. 동원문화사, 서울.
4 식품의약품안전처 (2007) 대한약전외한약(생약)규격집, 325, 429. 동원문화사, 서울.
5 한국약학대학협의회 약전분과회 (2008) 제9개정 대한약전 해설서 I, 103-105. 신일북스, 서울.
6 Oh, J. H., Sin, J. S., Ahn, E. S., Lee, S. J., Lee, J., Lim, J., Hong, S., Hong, J. and Lee, Y. J. (2009) A literature survey of the modern techniques used for the processing of herbal medicines. J. Kor. Pharm. Sci. 39: 275-297.
7 식품의약품안전처. 식품의약품안전청 고시 제2011-42호. 2011.
8 식품의약품안전처. (2009) 식품의약품안전청 고시 제2009-13호.
9 Ha, T. T., Cho, S. O., Ban, J. Y., Kim, J. Y., Ju, H. S., Koh, S. B., Song, K. S. and Seong, Y. H. (2008) Neuroprotective effect of Sanguisorbae Radix against oxidative stress-induced brain damage: in vitro and in vivo. Biol. Pharm. Bull. 31: 2028-2035.   DOI
10 Ayoub, N. A. (2003) Unique phenolic carboxylic acids from Sanguisorba minor. Phytochemistry. 63: 433-436.   DOI
11 Mimaki, Y., Fukushima, M., Yokosuka, A., Sashida, Y., Furuya, S. and Sakagami, H. (2001) Triterpene glycosides form the roots of Sanguisorba officinalis. Phytochemistry. 57: 773-779.   DOI
12 Liu, X., Shi, B. and Yu, B. (2004) Four new dimeric triterpene glucosides form Sanguisorba officinalis. Tetrahedron. 60: 11647-11654.   DOI
13 Chen, Y. and Liu H. (2008) Determination of hyperoside in Sanguisorba officinalis by RP-HPLC. Lishizhen Med. Materia Med. Res. 19: 455-456.
14 Sha, M., Cao, A., Wang, B., Liu, C., Geng, J. and Liu, W. (1998) Determination of hyperin in Sanguisorba officinalis L. by high performance liquid chromatography. Se. Pu. 16: 226-228.
15 Zhang, M., Zhu, S., Yan, Z., Fu, T. and Duan, M. (2009) HPLC determination of gallic acid and ellagic acid in Sanguisorba officinalis L. Modern Sci. Instrum. 5: 69-70.
16 Chen, X., Li, J., Li, Y. and Yi, L. (2011) Study on quality standard of Radix Sanguisorbae, Radix Sanguisorbae tablets and charcoal Radix Sanguisorbae. China Med. Herald. 8: 64-67.
17 동의학자료실 (1993) 동약법제, 13-53, 여강출판사, 서울.
18 Lee, B. C., Sim, G. S., Kim, J. H., Kim, J. H. and Pyo, H. B. (2006) Effect of the processed Selaginella tamariscina on antioxidation and inhibition of matrix metalloproteinase. J. Soc. Cosmet. Scientists Korea. 32: 69-74.
19 강병수, 서부일, 최호영. (2003) 한약포제와 임상응용. 202-205. 영림사, 서울.
20 Bae, K. H. (2008) The medicinal plant of Korea, 233. 교학사, 서울.
21 한방약리학교재편찬위원회 (2010) 한방약리학, 382-385. 신일북스, 서울.
22 생약학교재편찬위원회 (2012) 생약학, 193-195. 동명사, 서울.
23 Nguyen, T. T., Cho, S. O., Ban, J. Y., Kim, J. Y., Ju, H. S., Hoh, S. B., Song, K. S. and Seong, Y. H. (2008) Neuroprotective effect of Sanguisorbae Radix against oxidative stress-induced brain damage: in vitro and in vivo. Biol. Pharm. Bull. 31: 2028-2035.   DOI
24 Ban, J. Y., Nguyen, T. T., Lee, H. J., Cho, S. O., Ju, H. S., Kim, J. Y., Bae, K., Song, K. S. and Seong, Y. H. (2008) Neuroprotective properties of gallic acid from Sanguisorbae Radix on amyloid beta protein (25-35)-induced toxicity in cultured rat cortical neurons. Biol. Pharm. Bull. 31: 149-153.   DOI
25 Zhang, L., Koyyalamudi, S. R., Jeong, S. C., Reddy, N., Smith, P. T., Ananthan, R. and Longvah, T. (2012) Antioxidant and immunomodulatory activities of polysaccharides from the roots of Sanguisorba officinalis. Int. J. Biol. Macromol. 51: 1057-1062.   DOI
26 Tong, H., Mao, D., Zhai, M., Zhang, Z., Sun, G. and Jiang, G. (2015) Macrophage activation induced by the polysaccharides isolated from the roots of Sanguisorba officinalis. Pharm. Biol. 53: 1511-1515.   DOI
27 Shin, J. A., Kim, J. S., Kwon, K. H., Nam, J. S., Jung, J. Y., Cho, N. P. and Cho, S. D. (2012) Apoptotic effect of hot water extract of Sanguisorba officinalis L. in human oral cancer cells. Oncol. Lett. 4: 489-494.   DOI
28 Sun, W., Zhang, Z. L., Liu, X., Zhang, S., He, L., Wang, Z. and Wang, G. S. (2012) Terpene glycosides from the roots of Sanguisorba officinalis L. and their hemostatic activities. Molecules. 17: 7629-7636.   DOI
29 김재길 (1992) 임상응용 한약포제학. 97-98. 약업신문사 출판국, 서울.
30 Park, M. K., Park, J. H., Kim, J. M., Kim, W. Y. and Oh, J. Y. (1993) Study on the process of Sanguisorbae Radix. Anal. Sci. Technol. 6: 391-394.
31 Kang, T. L. and Park, M. K. (1994) Study on the process of Sanguisorbae Radix -Tomentosolic acid isolated from roasted Sanguisorbae Radix-. Yakhak Hoeji. 38: 712-714.
32 Kang, T. L. Hwang, G. S., Kim, J. M., Oh, J. Y., Park, J. H. and Park, M. K. (1994) Study on the process of Sanguisorbae Radix -Increased contents of pomolic acid and acetylpomolic acid by the process-. Yakhak Hoeji. 38: 782-785.
33 Seo, C. S., Kim, J. H., Shin, H. K., Hwang, S. Y. and Kim, B. S. (2014) Quantitative analysis of anthraquinones in Cassiae Semen by processing method. Kor. J. Pharmacogn. 45: 200-208.