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Anti-Obesity Effect of Isoegomaketone Isolated from Perilla frutescens (L.) Britt. cv. Leaves  

So, Yangkang (Korea Atomic Energy Research Insititute)
Jo, Yun Ho (Korea Atomic Energy Research Insititute)
Nam, Bo Mi (Korea Atomic Energy Research Insititute)
Lee, Seung Young (Korea Atomic Energy Research Insititute)
Kim, Jin-Baek (Korea Atomic Energy Research Insititute)
Kang, Si-Yong (Korea Atomic Energy Research Insititute)
Jeong, Hye Gwang (Colleage of Phamacy, Chungnam Natinal University)
Jin, Chang Hyun (Korea Atomic Energy Research Insititute)
Publication Information
Korean Journal of Pharmacognosy / v.46, no.4, 2015 , pp. 283-288 More about this Journal
Abstract
In this study, we investigated anti-obesity effect of isoegomaketone (IK) isolated from leaves extract of Perilla frutescens (L.) Britt. cv. We verified differentiation and lipid accumulation by Oil Red O staining in 3T3-L1 cells after IK treatment with differentiation media. IK inhibited mRNA expression of adipocyte specific genes that were related with differentiation of 3T3-L1 cells. We confirmed the effects of IK on body weight and visceral fat mass in obese mice. Mice were randomly divided into three groups; normal diet group (ND), high-fat diet group (HFD) and high-fat diet with IK group (HFD-IK). The obesity mice were induced by feeding the 45% high-fat diet to the C57BL/6J mice during 4 weeks. After HFD-IK was orally administered 10 mg/kg of IK. As a result, the body weight of HFD and HFD-IK was increased 2.4 times and 1.7 times of ND, respectively. Also visceral fat mass of HFD was increased 24 times but in the case of HFD-IK was increased to 13 times in comparison with ND. Taken together, our findings suggest that IK reduced differentiation and adiogenesis in 3T3-L1 cells, decreased the body weight and visceral fat mass in obesity mice. These results suggest that IK may have a potential benefit as anti-obesity material.
Keywords
Isoegomaketone; Anti-obesity; 3T3-L1 cells; C57BL/6J; High-fat diet;
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