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Quantitative Analysis of (+)-Catechin and Diosgenin from Smilax china L. Rhizome  

Yin, Jun (Laboratory of Pharmacognosy, College of Pharmacy, Chung-Ang University)
Yoon, Ki Hoon (Laboratory of Pharmacognosy, College of Pharmacy, Chung-Ang University)
Hwang, Yoon Jeong (Laboratory of Pharmacognosy, College of Pharmacy, Chung-Ang University)
Lee, Ji Yeon (Laboratory of Pharmacognosy, College of Pharmacy, Chung-Ang University)
Shin, Hwa Sup (Department of Biomedical Chemistry, Konkuk University)
Lee, Min Won (Laboratory of Pharmacognosy, College of Pharmacy, Chung-Ang University)
Publication Information
Korean Journal of Pharmacognosy / v.46, no.3, 2015 , pp. 189-194 More about this Journal
Abstract
Validation and contents determination of (+)-catechin and diosgenin from Smilax china L. rhizome (SC) were confirmed using Ultra-Performance Liquid Chromatography (UPLC). (+)-Catechin was isolated from 60% prethanol SC extract and diosgenin was isolated by acid hydrolysis of saponin fraction from 60% prethanol extract of SC. Finally we have established the validation of the isolated compounds [(+)-catechin, diosgenin] and contents determinations of (+)-catechin and diosgenin by UPLC on 60% prethanol extract of SC [(+)-catechin: 0.06878%, diosgenin: 0.48169%], and on hot water extract of SC [(+)-catechin: 0.06584%, diosgenin: 0.42178%].
Keywords
Smilax china L.; (+)-Catechin; Diosgenin; UPLC; Acid hydrolysis; Validation; Content;
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