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Effects of MeOH Extract of Impatiens balsamina L. on the Metabolism of Amyloid Precursor Protein in Neuroblastoma Cells  

Jo, Yoon Jeong (College of Pharmacy, Woosuk University)
Leem, Jae Yoon (College of Pharmacy, Woosuk University)
Publication Information
Korean Journal of Pharmacognosy / v.46, no.1, 2015 , pp. 72-77 More about this Journal
Abstract
One of the most common forms of dementia, Alzheimer's disease (AD) is a progressive neurodegenerative disorder symptomatically characterized by impairment in memory and cognitive abilities. AD is characterized pathologically by the presence of intracellular neurofibrillary tangles and deposition of ${\beta}$-amyloid ($A{\beta}$) peptides, believed to be neurotoxic and now is also considered to have a role on the mechanism of memory dysfunction. In this study, we tested that MeOH extract of Impatiens balsamina L. (IBM) affects on the processing of APP from the APPswe over-expressing Neuro2a cell line. We found that IBM increased over 2 folds of the $sAPP{\alpha}$ secretion level, a main metabolite of ${\alpha}$-secretase. We shown that IBM reduced the secretion level of $A{\beta}42$ and $A{\beta}40$ without cytotoxicity. BACE (${\beta}$-site APP cleaving enzyme) FRET assay shown that BACE activity was specifically decreased in the presence of IBM. We suggest that Impatiens balsamina L. may be an useful source to develop a herbal medicine of BACE inhibitor for Alzheimer's disease.
Keywords
Alzheimer's disease; ${\beta}$-Amyloid peptides; Impatiens balsamina L.; APPswe; BACE;
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