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Simultaneous Determination of Platyphylloside, Aceroside VIII and Betulin in Betula platyphylla bark by HPLC-DAD  

Cho, NamKi (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Kim, Dae Hyun (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Sung, Sang Hyun (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Publication Information
Korean Journal of Pharmacognosy / v.45, no.4, 2014 , pp. 294-299 More about this Journal
Abstract
The simultaneous determination of platyphylloside, aceroside and betulin was established for the quality control of Betula platyphylla bark using a high performance liquid chromatography and diode-array UV/Vis detector (HPLC-DAD). Separation and quantification were successfully achieved with a INNO C18 column ($5{\mu}m$, 4.6 mm $I.D.{\times}150mm$) by gradient elution of a mixture of methanol and water at a flow rate of 1.0 ml/min. Validation of the developed method was performed by various factor such as linearity, specificity, precision, accuracy, system suitability and stability. This method was successfully applied to the determination of contents of platyphylloside, aceroside VIII and betulin in three batches of Betula platyphylla bark extract. These results suggest that the developed HPLC method is simple, effective and could be utilized as a quality control method for Betula platyphylla bark products.
Keywords
Betula platyphylla bark; HPLC-DAD; Validation; Quantification;
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