Browse > Article

Effect of Clitocybin A on the Proliferation of Dermal Papilla Cells  

Kang, Jung-Il (Department of Medicine, School of Medicine, Jeju National University)
Kim, Min-Kyoung (Department of Medicine, School of Medicine, Jeju National University)
Yoo, Eun-Sook (Department of Medicine, School of Medicine, Jeju National University)
Yoo, Ick-Dong (Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kang, Hee-Kyoung (Department of Medicine, School of Medicine, Jeju National University)
Publication Information
Korean Journal of Pharmacognosy / v.45, no.4, 2014 , pp. 288-293 More about this Journal
Abstract
The present study was conducted to evaluate the hair growth-promoting effect of Clitocybin A from mushroom Clitocybe aurantiaca with dermal papilla cells (DPCs), which play important roles in the regulation of hair cycle. Clitocybin A significantly increased the proliferation of immortalized rat vibrissa DPCs. Flow cytometry analysis revealed that Clitocybin A promoted cell-cycle progression through G0/G1 to S phase in immortalized rat vibrissa DPCs. In addition, Clitocybin A increased the level of cell cycle proteins such as cyclin D1, phospho-pRB, and phospho-CDK2. To elucidate the molecular mechanisms of Clitocybin A on the proliferation of DPCs, we examined the activation of wnt/${\beta}$-catenin signaling which is known to regulate hair follicle development, differentiation and hair growth. Clitocybin A activated wnt/${\beta}$-catenin signaling via the increase of phospho(ser552)-${\beta}$-catenin, phospho(ser675)-${\beta}$-catenin and phospho(ser9)-$GSK3{\beta}$. Furthermore, Clitocybin A markedly increased the activation of extracellular signal-regulated kinase (ERK). These results suggest that the Clitocybin A may induce hair growth by proliferation of DPCs via cell-cycle progression as well as the activation of Wnt/${\beta}$-catenin signaling and ERK pathway.
Keywords
Hair growth; RAF; Dermal papilla cells; Cell cycle; Wnt/${\beta}$-catenin; ERK;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kwack, M. H., Kang, B. M., Kim, M. K., Kim, J. C. and Sung, Y. K. (2011) Minoxidil activates beta-catenin pathway in human dermal papilla cells: A possible explanation for its anagen prolongation effect. J. Dermatol. Sci. 62: 154-159.   DOI   ScienceOn
2 Price, V. H. (1999) Treatment of hair loss. N. Engl. J. Med. 341: 964-973.   DOI   ScienceOn
3 Ellis, J. A., Sinclair, R. and Harrap, S. B. (2002) Androgenetic alopecia: pathogenesis and potential for therapy. Expert. Rev. Mol. Med. 4: 1-11.
4 Cotsarelis, G. and Millar, S. E. (2001) Towards a molecular understanding of hair loss and its treatment. Trends Mol. Med. 7: 293-301.   DOI   ScienceOn
5 Kaufman, K. D. and Dawber, R. P. (1999) Finasteride, a Type 2 5alpha-reductase inhibitor, in the treatment of men with androgenetic alopecia. Expert. Opin. Investig. Drugs. 8: 403-415.   DOI
6 Kaufman, K. D. (2002) Androgens and alopecia. Mol. Cell Endocrinol. 198: 89-95.   DOI   ScienceOn
7 Hamaoka, H., Minakuchi, K., Miyoshi, H., Arase, S., Chen, C. H. and Nakaya, Y. (1997) Effect of $K^+$ channel openers on $K^+$ channel in cultured human dermal papilla cells. J. Med. Invest. 44: 73-77.
8 Shorter, K., Farjo, N. P., Picksley, S. M. and Randall, V. A. (2008) Human hair follicles contain two forms of ATP-sensitive potassium channels, only one of which is sensitive to minoxidil. FASEB J. 22: 1725-1736.   DOI   ScienceOn
9 Han, J. H., Kwon, O. S., Chung, J. H., Cho, K. H., Eun, H. C. and Kim, K. H. (2004) Effect of minoxodil on proliferation and apoptosis in dermal papilla cells of human hair follicle. J. Dermatol. Sci. 34: 91-98.   DOI   ScienceOn
10 Ito, M., Yang, Z., Andl, T., Cui, C., Kim, N., Millar, S. E. and Cotsarelis, G. (2007) Wnt-dependent de novo hair follicle regeneration in adult mouse skin after wounding. Nature 447: 316-320.   DOI   ScienceOn
11 Monick, M. M., Carter, A. B., Robeff, P. K., Flaherty, D. M., Peterson, M. W. and Hunninghake, G. W. (2001) Lipopolysaccharide activates Akt in human alveolar macrophages resulting in nuclear accumulation and transcriptional activity of beta-catenin. J. Immunol. 166: 4713-4720.   DOI
12 Greco, V., Chen, T., Rendl, M., Schober, M., Pasolli, H. A., Stokes, N., Dela Cruz-Racelis, J. and Fuchs, E. (2009) A two-step mechanism for stem cell activation during hair regeneration. Cell Stem Cell 4: 155-169.   DOI   ScienceOn
13 Johnson, D. G. and Walker, C. L. (1999) Cyclins and cell cycle checkpoints. Annu. Rev. Pharmacol. Toxicol. 39: 295-312.   DOI   ScienceOn
14 Hedgepeth, C. M., Conrad, L. J., Zhang, J., Huang, H. C., Lee, V. M. and Klein, P. S. (1997) Activation of the Wnt signaling pathway: a molecular mechanism for lithium action. Dev. Biol. 185: 82-91.   DOI   ScienceOn
15 Hino, S., Tanji, C., Nakayama, K. I., and Kikuchi, A. (2005) Phosphorylation of beta-catenin by cyclic AMP-dependent protein kinase stabilizes beta-catenin through inhibition of its ubiquitination. Mol. Cell. Biol. 25: 9063-9072.   DOI   ScienceOn
16 Stenn, K. S. and Paus, R. (2001) Controls of hair follicle cycling. Physiol. Rev. 81: 449-494.
17 Sherr, C. J. (1996) Cancer cell cycles. Science 274: 1672-1677.   DOI   ScienceOn
18 Sherr, C. J. and Roberts, J. M. (1999) CDK inhibitors: Positive and negative regulators of G1-phase progression. Genes Dev. 13: 1501-1512.   DOI   ScienceOn
19 Prall, O. W., Sarcevic, B., Musgrove, E. A., Watts, C. K. and Sutherland, R. L. (1997) Estrogen-induced activation of Cdk4 and Cdk2 during G1-S phase progression is accompanied by increased cyclin D1 expression and decreased cyclin-dependent kinase inhibitor association with cyclin E-Cdk2. J. Biol. Chem. 272: 10882-10894.   DOI   ScienceOn
20 Tetsu, O. and McCormick, F. (1999) Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells. Nature 398: 422-426.   DOI   ScienceOn
21 Han, S. K., Cho, J. W., Cho, H. J., Kim, H. J. and Lee, Y. M. (2013) A field guide to mushrooms. 2th Ed. Korea national arboretum, Hwang TS, pp. 386-393. Geobook, Seoul
22 Kim, Y. H., Cho, S. M., Hyun, J. W., Ryoo, I. J., Choo, S. J., Lee, S., Seok, S. J, Hwang, J. S., Sohn, E. D., Yun, B. S., Bae, K. H. and Yoo, I. D. (2008) A new oxidant, clitocybin A, from the culture broth of Clitocybe aurantiaca. J. Antibiot. 61: 573-576.   DOI   ScienceOn
23 Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.   DOI   ScienceOn
24 Moon, E. Y., Kim, Y. H., Ryoo, I. J. and Yoo, I. D. (2009) Clitocybins, novel isoindolinone free radical scavengers, from mushroom Clitocybe aurantiaca inhibit apoptotic cell death and cellular senescence. Biol. Pharm. Bull. 32: 1689-1694.   DOI
25 Filsell, W., Little, J. C., Stones, A. J., Granger, S. P. and Bayley, S. A. (1994) Transfection of rat dermal papilla cells with a gene encoding a temperature-sensitive polyomavirus large T antigen generates cell lines a differentiated phenotype. J. Cell Sci. 107: 1761-1772.
26 Carmichael, J., DeGraff, W. G., Gazdar, A. F., Minna, J. D. and Mitchell, J. B. (1987) Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47: 936-942.
27 Jahoda, C. A., Horne, K. A. and Oliver, R. F. (1984) Induction of hair growth by implantation of cultured dermal papilla cells. Nature 311: 560-562.   DOI   ScienceOn
28 Horne, K. A., Jahoda, C. A. and Oliver, R. F. (1986) Whisker growth induced by implantation of cultured vibrissa dermal papilla cells in the adult rat. J. Embryol. Exp. Morphol. 97: 111-124.
29 Wangefjord, S., Brandstedt, J., Ericson Lindquist, K., Nodin, B., Jirstrom, K. and Eberhard, J. (2013) Associations of beta-catenin alterations and MSI screening status with expression of key cell cycle regulating proteins and survival from colorectal cancer. Diagn. Pathol. 8: 10.   DOI
30 Lavoie, J. N., L'Allemain, G., Brunet, A., Muller, R. and Pouyssegur, J. (1996) Cyclin D1 expression is regulated positively by the p42/p44MAPK and negatively by the p38/HOGMAPK pathway. J. Biol. Chem. 271, 20608-20616.   DOI   ScienceOn
31 Brudvik, K. W., Paulsen, J. E., Aandahl, E. M., Roald, B. and Tasken, K. (2011) Protein kinase A antagonist inhibits ${\beta}$-catenin nuclear translocation, c-Myc and COX-2 expression and tumor promotion in Apc(Min/+) mice. Mol. Cancer. 10: 149.   DOI
32 Chuang, S. M., Wang, I. C. and Yang, J. L. (2000) Roles of JNK, p38 and ERK mitogen-activated protein kinases in the growth inhibition and apoptosis induced by cadmium. Carcinogenesis 21: 1423-1432.   DOI   ScienceOn
33 Park, E. S., Yoo, K. D., Kang, S. I., Yoo, S. H., Won, H. H., Kim, Y. H., Yoo, I. D., Yoo, H. S., Hong, J. T. and Yun, Y. P. (2012) Clitocybin A, a novel isoindolinone, from mushroom Clitocybe aurantiaca, inhibits cell proliferation through G1 phase arrest by regulating PI3K/Akt cascade in vescular smooth muscle cells. J. Pharmacological Sci. 118: 171-177.   DOI
34 Ouji, Y., Yoshikawa, M., Moriya, K. and Ishizaka, S. (2007) Effects of Wnt-10b on hair shaft growth in hair follicle cultures. Biochem. Biophys. Res. Commun. 359: 516-522.   DOI   ScienceOn