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Isolation and Identification of Phenol Compounds from Acer tegmentosum and their Anti-inflammatory Activity  

Song, Na-Young (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine)
Lee, Kwang Jin (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine)
Ma, Jin Yeul (KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine)
Publication Information
Korean Journal of Pharmacognosy / v.45, no.2, 2014 , pp. 93-100 More about this Journal
Abstract
The Acer tegmentosum (3 kg) were extracted with boiled water and the freeze dried extract powder was partitioned with $CH_2Cl_2$, EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc and n-BuOH fraction, six phenolic compounds were isolated through the silica gel, octadecyl silica gel and sephadex LH-20 column chromatography. On the basis of spectroscopic methods, such as $^1H$-NMR and $^{13}C$-NMR, and LC/MS, the chemical structures of the compounds as feniculin (1), avicularin (2), (+)-catechin (3), (-)-epicatechin (4), salidroside (5) and 6'-O-galloylsalidroside (6). In this study, compounds 1 and 2 have been first isolated from the A. tegmentosum. To provide insight into the effects of six compounds isolated from A. tegmentosum on inflammation, we investigated its effect on nitric oxide (NO) production in RAW 264.7 cells using lipopolysaccharide (LPS) stimulation. Compounds 1 and 6 slightly repressed NO production. Also, compounds 3 and 4 inhibited NO secretion with statistical significance. However, compounds 2 and 5 did not show any inhibitory effect on NO production.
Keywords
Acer tegmentosum; Anti-inflammation; Avicularin; Feniculin; Isolation; NMR;
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