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Anti-neuroinflammatory Effects of Quercetin-3-O-glucuronide Isolated from the Leaf of Vitis labruscana on LPS-induced Neuroinflammation in BV2 Cells  

Yoon, Chi-Su (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Kim, Dong-Cheol (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Ko, Won-Min (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Kim, Kyoung-Su (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Lee, Dong-Sung (Hanbang Body-Fluid Research Center, Wonkwang University)
Kim, Dae-Sung (Hanpoong Pharm & Foods Co., Ltd.)
Cho, Hyoung-Kwon (Hanpoong Pharm & Foods Co., Ltd.)
Seo, Jungwon (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University)
Kim, Sung Yeon (Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University)
Oh, Hyuncheol (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Kim, Youn-Chul (Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University)
Publication Information
Korean Journal of Pharmacognosy / v.45, no.1, 2014 , pp. 17-22 More about this Journal
Abstract
Grapes has long been used for food, and reported as containing polyphenol which has antioxidant and anti-cancer effects. Neuroinflammation is chronic inflammation at the brain, lead to neurodegenerative diseases. In this study, quercetin-3-O-glucuronide (QG) isolated from the leaf of Vitis labruscana has anti-neuroinflammatory effects. QG were investigated using MTT assay, western blot, nitric oxide (NO) assay, prostaglandin $E_2$ ($PGE_2$) assay, cytokine assay in lipopolysaccharide (LPS)-induced inflammation in BV2 cells. QG dose-dependently attenuated the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), accordingly inhibited the production of NO and $PGE_2$. QG decreases the levels of proinflammatory cytokine such as tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interlukin-$1{\beta}$ (IL-$1{\beta}$). Thereby, QG may offer therapeutic potential for treatment of neurodegenerative disease related to neuroinflammation.
Keywords
Vitis labruscana leaf; Quercetin-3-O-glucuronide; Anti-neuroinflammation; BV2 cells;
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