Browse > Article

The Hair Growth Effects of Wheat Bran  

Kang, Jung-Il (Department of Medicine, School of Medicine, Jeju National University)
Moon, Jungsun (Department of Medicine, School of Medicine, Jeju National University)
Kim, Eun-Ji (Department of Medicine, School of Medicine, Jeju National University)
Lee, Young-Ki (Department of Medicine, School of Medicine, Jeju National University)
Koh, Young-Sang (Department of Medicine, School of Medicine, Jeju National University)
Yoo, Eun-Sook (Department of Medicine, School of Medicine, Jeju National University)
Kang, Hee-Kyoung (Department of Medicine, School of Medicine, Jeju National University)
Yim, Dongsool (College of Pharmacy, Sahmyook University)
Publication Information
Korean Journal of Pharmacognosy / v.44, no.4, 2013 , pp. 384-390 More about this Journal
Abstract
This study was conducted to evaluate the effect of wheat bran on the promotion of hair growth. When rat vibrissa follicles were treated with n-hexane fraction of wheat bran, the hair-fiber lengths of the vibrissa follicles significantly increased. Moreover, n-hexane fraction of wheat bran was found to significantly induce the telogen-anagen transition in C57BL/6 mice. The fraction increased the proliferation of immortalized vibrissa dermal papilla cells (DPCs) in a dose dependent manner. To elucidate the molecular mechanisms in relation to proliferation of DPCs by the fraction of wheat bran, we examined the expression of cell cycle proteins and wnt/${\beta}$-catenin signaling components. Western blot analysis revealed that the proliferation of DPC by n-hexane fraction of wheat bran was accompanied by increased the level of cyclin D1, cyclin E, phospho-CDK2 and phospho-pRB. In addition, the fraction of wheat bran increased the level of phospho(ser552)-${\beta}$-catenin, phospho(ser675)-${\beta}$-catenin and phospho(ser9)-GSK$3{\beta}$. These results suggest that the hair growing potential of wheat bran mediated by proliferation of DPCs via the regulation of cell cycle proteins and Wnt/${\beta}$-catenin signaling.
Keywords
Hair growth; Wheat bran; C57BL/6 mice; Dermal papilla cells; Wnt/${\beta}$-catenin;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Shewry, P. (2009) The Healthgrain programme opens new opportunities for improving wheat for nutrition and health. Nutr. Bull. 34: 225-231.   DOI   ScienceOn
2 Stevenson, L., Phillips, F., O'Sullivan, K. and Walton, J. (2012) Wheat bran: its composition and benefits to health, a European perspective. Int. J. Food Sci. Nutr. 63: 1001-1013.   DOI   ScienceOn
3 Yu, L., Haley, S., Perret, J., Harris, M., Wilson, J. and Qian, M. (2002) Free radical scavenging properties of wheat extracts. J. Agric. Food Chem. 50: 1619-1624.   DOI   ScienceOn
4 Kim, K. H., Tsao, R., Yang, R. and Cui, S. W. (2006) Phenolic acid profiles and antioxidant activities of wheat bran extracts and the effect of hydrolysis conditions. Food Chem. 95: 466-473.   DOI   ScienceOn
5 Shan, Y., Cheng, Y., Zhang, Y., Guan, F. Q., Sun, H., Ren, X. C., Chen, Y., Feng, X. and Yang, J. M. (2013) Triticuside a, a dietary flavonoid, inhibits proliferation of human breast cancer cells via inducing apoptosis. Nutr. Cancer 65: 891-899.
6 Price, V. H. (1999) Treatment of hair loss. N. Engl. J. Med. 341: 964-973.   DOI   ScienceOn
7 Ellis, J. A., Sinclair, R. and Harrap, S. B. (2002) Androgenetic alopecia: pathogenesis and potential for therapy. Expert. Rev. Mol. Med. 4: 1-11.
8 Cotsarelis, G. and Millar, S. E. (2001) Towards a molecular understanding of hair loss and its treatment. Trends Mol. Med. 7: 293-301.   DOI   ScienceOn
9 Kaufman, K. D. and Dawber, R. P. (1999) Finasteride, a Type 2 5alpha-reductase inhibitor, in the treatment of men with androgenetic alopecia. Expert. Opin. Investig. Drugs. 8: 403-415.   DOI
10 Kaufman, K. D. (2002) Androgens and alopecia. Mol. Cell Endocrinol. 198: 89-95.   DOI   ScienceOn
11 Hamaoka, H., Minakuchi, K., Miyoshi, H., Arase, S., Chen, C. H. and Nakaya, Y. (1997) Effect of $K^{+}$ channel openers on $K^{+}$ channel in cultured human dermal papilla cells. J. Med. Invest. 44: 73-77.
12 Shorter, K., Farjo, N. P., Picksley, S. M. and Randall, V. A. (2008) Human hair follicles contain two forms of ATP-sensitive potassium channels, only one of which is sensitive to minoxidil. FASEB J. 22: 1725-1736.   DOI   ScienceOn
13 Lachgar, S., Charveron, M., Gall, Y. and Bonafe, J. L. (1998) Minoxidil upregulates the expression of vascular endothelial growth factor in human hair dermal papilla cells. Br. J. Dermatol. 138: 407-411.   DOI   ScienceOn
14 Kwack, M. H., Kang, B. M., Kim, M. K., Kim, J. C. and Sung, Y. K. (2011) Minoxidil activates beta-catenin pathway in human dermal papilla cells: A possible explanation for its anagen prolongation effect. J. Dermatol. Sci. 62: 154-159.   DOI   ScienceOn
15 Han, J. H., Kwon, O. S., Chung, J. H., Cho, K. H., Eun, H. C. and Kim, K. H. (2004) Effect of minoxodil on proliferation and apoptosis in dermal papilla cells of human hair follicle. J. Dermatol. Sci. 34: 91-98.   DOI   ScienceOn
16 Johnson, D. G. and Walker, C. L. (1999) Cyclins and cell cycle checkpoints. Annu. Rev. Pharmacol. Toxicol. 39: 295-312.   DOI
17 Tetsu, O. and McCormick, F. (1999) Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells. Nature 398: 422-426.   DOI   ScienceOn
18 Hedgepeth, C. M., Conrad, L. J., Zhang, J., Huang, H. C., Lee, V. M, and Klein, P. S. (1997) Activation of the Wnt signaling pathway: a molecular mechanism for lithium action. Dev. Biol. 185: 82-91.   DOI   ScienceOn
19 Hino, S., Tanji, C., Nakayama, K. I., and Kikuchi, A. (2005) Phosphorylation of beta-catenin by cyclic AMP-dependent protein kinase stabilizes beta-catenin through inhibition of its ubiquitination. Mol. Cell. Biol. 25: 9063-9072.   DOI   ScienceOn
20 Monick, M. M., Carter, A. B., Robeff, P. K., Flaherty, D. M., Peterson, M. W., and Hunninghake, G. W. (2001) Lipopolysaccharide activates Akt in human alveolar macrophages resulting in nuclear accumulation and transcriptional activity of beta-catenin. J. Immunol. 166: 4713-4720.   DOI
21 Matsuda, H., Yamazaki, M., Asanuma, Y., and Kubo, M. (2003) Promotion of Hair Growth by Ginseng Radix on Cultured Mouse Vibrissal Hair Follicles. Phytother. Res. 17: 797-800.   DOI   ScienceOn
22 Ohnemus, U., Uenalan, M., Conrad, F., Handjiski, B., Mecklenburg, L., Nakamura, M., Inzunza, J., Gustafsson, J.A. and Paus, R. (2005) Hair cycle control by estrogens: Catagen induction via estrogen receptor (ER)-alpha is checked by ER beta signaling. Endocrinology 146: 1214-1225.   DOI   ScienceOn
23 Filsell, W., Little, J. C., Stones, A. J., Granger, S. P. and Bayley, S. A. (1994) Transfection of rat dermal papilla cells with a gene encoding a temperature-sensitive polyomavirus large T antigen generates cell lines a differentiated phenotype. J. Cell Sci. 107: 1761-1772.
24 Carmichael, J., DeGraff, W. G., Gazdar, A. F., Minna, J. D. and Mitchell, J. B. (1987) Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47: 936-942.
25 Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.   DOI   ScienceOn
26 Philpott, M. P., Green, M. R. and Kealy, T. (1992) Rat hair follicle growth in vitro. Br. J. Dermatol. 127: 600-607.   DOI   ScienceOn
27 Philpott, M. P. and Kealey, T. (2000) Cyclical changes in rat vibrissa follicles maintained in vitro. J. Invest. Dermatol. 115: 1152-1155.   DOI   ScienceOn
28 Müller-Röver, S., Handjiski, B., van der Veen, C., Eichmüller, S., Foitzik, K., McKay, I. A., Stenn, K. S. and Paus, R. (2001) A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages. J. Invest. Dermatol. 117: 3-15.   DOI   ScienceOn
29 Kaufman, K. D., Olsen, E. A., Whiting, D., Savin, R., DeVillez, R., Bergfeld, W., Price, V. H., van Neste, D., Roberts, J. L., Hordinsky, M. Shapiro, J., Binkowitz, B. and Gormley GJ. (1998) Finasteride in the treatment of men with androgenetic alopecia. J. Am. Acad. Dermatol. 39: 578-589.   DOI   ScienceOn
30 Kang, J. I., Kim, E. J., Kim, M. K., Jeon, Y. J., Kang, S. M., Koh, Y. S., Yoo, E. S. and Kang, H.K. (2013) The promoting effect of Ishige sinicola on hair growth. Mar. Drugs 11: 1783- 1799.   DOI   ScienceOn
31 Jahoda, C. A., Horne, K. A. and Oliver, R. F. (1984) Induction of hair growth by implantation of cultured dermal papilla cells. Nature 311: 560-562.   DOI   ScienceOn
32 Horne, K. A., Jahoda, C. A. and Oliver, R. F. (1986) Whisker growth induced by implantation of cultured vibrissa dermal papilla cells in the adult rat. J. Embryol. Exp. Morphol. 97: 111-124.
33 Sherr, C. J. (1996) Cancer cell cycles. Science 274: 1672- 1677.   DOI   ScienceOn
34 Sherr, C. J. and Roberts, J. M. (1999) CDK inhibitors: Positive and negative regulators of G1-phase progression. Genes Dev. 13: 1501-1512.   DOI   ScienceOn
35 Prall, O. W., Sarcevic, B., Musgrove, E. A., Watts, C. K. and Sutherland, R. L. (1997) Estrogen-induced activation of Cdk4 and Cdk2 during G1-S phase progression is accompanied by increased cyclin D1 expression and decreased cyclin-dependent kinase inhibitor association with cyclin E-Cdk2. J. Biol. Chem. 272: 10882-10894.   DOI   ScienceOn
36 Wangefjord, S., Brandstedt, J., Ericson Lindquist, K., Nodin, B., Jirstrom, K. and Eberhard, J. (2013) Associations of betacatenin alterations and MSI screening status with expression of key cell cycle regulating proteins and survival from colorectal cancer. Diagn. Pathol. 8: 10.   DOI
37 Brudvik, K. W., Paulsen, J. E., Aandahl, E. M., Roald, B. and Taskén, K. (2011) Protein kinase A antagonist inhibits $\beta$-catenin nuclear translocation, c-Myc and COX-2 expression and tumor promotion in Apc(Min/+) mice. Mol. Cancer. 10: 149.
38 Lee, S. H., Yoon, J., Shin, S. H., Zahoor, M., Kim, H. J., Park, P. J., Park, W. S., Min, do. S., Kim, H. Y., and Choi, K. Y. (2012) Valproic acid induces hair regeneration in murine model and activates alkaline phosphatase activity in human dermal papilla cells. PLoS One. 7: e34152.   DOI   ScienceOn