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Simultaneous Analysis of the Chemical Compounds in Ojeok-san and Its Antioxidative Activity  

Kim, Seong-Sil (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Kim, Jung-Hoon (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Kim, Ohn Soon (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Kim, Yeji (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Shin, Hyeun-Kyoo (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Seo, Chang-Seob (Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine)
Publication Information
Korean Journal of Pharmacognosy / v.44, no.4, 2013 , pp. 362-367 More about this Journal
Abstract
Ojeok-san has been commonly used to treat low back pain disease. We performed the experiments on simultaneous analysis of 11 compounds and antioxidant effects of Ojeok-san. A HPLC method was established for simultaneous analysis of 11 compounds. The detection wavelengths were set at 230, 254, 280, 320, and 330 nm. The detected 11 compounds from Ojeok-san water extract showed good linearity($r^2{\geq}0.9997$). Limit of detection(LOD) and limit of quantification(LOQ) were 0.04-0.87 ${\mu}g/mL$ and 0.13-2.63 ${\mu}g/mL$, respectively. The antioxidant effects of Ojeok-san water extract were investigated by DPPH and ABTS assays. Ojeok-san water extract significantly increased the DPPH and ABTS radical scavenging effects in a dose-dependent manner. The $RC_{50}$ value of Ojeok-san on DPPH radical was 284.71 ${\mu}g/mL$ and that of ABTS radical was 96.16 ${\mu}g/mL$.
Keywords
Ojeok-san; HPLC; DPPH; ABTS; Simultaneous analysis; Antioxidant effect;
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