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Rhus Trichocarpa Suppresses IgE-mediated Allergic Response In Vitro and In Vivo  

Lim, Hannah (College of Pharmacy, Duksung Women's University)
Kim, Young Mi (College of Pharmacy, Duksung Women's University)
Publication Information
Korean Journal of Pharmacognosy / v.44, no.2, 2013 , pp. 118-124 More about this Journal
Abstract
Mast cells is the key effector cells for IgE-mediated allergic responses. In this study, we investigated whether Rhus trichocarpa extract (RT) inhibited IgE-mediated allergic responses in mast cells and an allergic animal model. We further tried to find its mechanism of action in mast cells. We found that RT suppressed antigen-stimulated degranulation and production of TNF-${\alpha}$ and IL-4 in rat basophilic leukemia (RBL)-2H3 mast cells and bone marrow-derived mast cells (BMMC), as well as IgE-mediated passive cutaneous anaphylaxis (PCA) in mice. As the mechanism of action of RT, it inhibited the activation of spleen tyrosine kinase (Syk), a pivotal signaling molecule for activation of mast cells and that of LAT, a downstream adaptor molecule of Syk in $Fc{\varepsilon}RI$-mediated signal pathways. RT also suppressed the activation of mitogen-activated protein (MAP) kinases and Akt. The current results demonstrated for the first time that RT has the anti-allergic effect through inhibiting degranulation and secretion of cytokines by suppression of Syk in antigen-stimulated mast cells. Therefore, RT might be useful for allergic diseases.
Keywords
Rhus trichocarpa; Allergy; Mast cells; Syk; Passive cutaneous anaphylaxis;
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