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Analysis of Active Components in Cirsium japonicum var. ussuriense Extracts and Their Effect on TGF-beta Induced Hepatic Stellate Cells Activation  

Kim, Seon-Young (Jeonju Biomaterials Institute)
Kim, Sang-Jun (Jeonju Biomaterials Institute)
Choi, Young Ji (Jeonju Biomaterials Institute)
Yu, Kang-Yeol (Jeonju Biomaterials Institute)
Chung, Chang-Ho (Jeonju Biomaterials Institute)
Shim, Jae-Suk (Imsil Herbal Medicine Association)
Jang, Seon-Il (College of Alternative Medicine, Jeonju University)
Yu, Dong-Hyun (Jeollabukdo ARES Specialization Crop Research Institute)
Jeong, Seung-Il (Jeonju Biomaterials Institute)
Publication Information
Korean Journal of Pharmacognosy / v.44, no.2, 2013 , pp. 110-117 More about this Journal
Abstract
Cirsium japonicum (CJ) leaf (L) alcoholic extracts were investigated for analysis their active components (flavonoids and flavanolignans; silymarins) and inhibitory effect on transforming growth factor (TGF)-${\beta}$ induced hepatic stellate cells (HSCs, LX-2 cells) activation. The CJ root (R) extracts were also analyzed and compared with leaf extracts. Total flavonoid and polyphenol contents of the leaf extracts showed higher than those of the root extracts. The content of each flavonoid compound, which was analyzed by HPLC, in CJ-L extracts was also higher than in CJ-R extracts. The results of flavanolignans content in CJ-L and CJ-R extracts were consistent in flavonoid and polyphenol. We studied inhibitory effect of two extracts against TGF-${\beta}1$ induced HSCs activation. The CJ-L extracts significantly suppressed overexpression of profibrogenic factor, ${\alpha}$-smooth muscle actin and collagen-${\alpha}1$(I). The CJ-R extract also showed inhibitory effect on TGF-${\beta}1$ induced HSCs activation, but the efficacy was lower than in CJ-L extract. These results suggest that CJ-L may contribute to the fibrotic liver treatment.
Keywords
Cirsium japonicum; Flavonoids; Flavonolignans; Hepatic stellate cells;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 통계청 (2011) 사망원인통계.
2 Tsukada, S., Parosns, C. J. and Ripe, R. A. (2006) Mechanism of liver fibrosis. Clin. Chim. Acta. 364: 33-60.   DOI   ScienceOn
3 Pinzani, M. (1995) Hepatic stellate (Ito) cells: expanding roles for a liver specific pericyte. J. Hepatol. 22: 700-706.   DOI   ScienceOn
4 Friedman, S. L., Roll, J. F., Boyles, J. and Bissell, D. M. (1985) Hepatic lipocytes. the principal collagen producing cells of normal rat liver. Proc. Natl. Acad. Sci. USA. 82: 8681-8685.   DOI   ScienceOn
5 Bedossa, P., Houglum, K., Trautwein, C., Holstege, A. and Chojkier, M. (1994) Stimulation of collagen a1(I) gene expres-sion is associated with lipid peroxidation in hepatocellular injury: a link to tissue fibrosis. Hepatology 19: 1262-1271.
6 Ishida, H., Umino, T., Tsuji, K. and Kosuge, T. (1987) Studies on antihemmorhagic substances in Herbs classified as hemostatics in Chinese medicine. VII. On the anthihmorrhagic principle in Cirsium japonicum DC. Chem. Pharm. Bull. 35: 861-864.   DOI   ScienceOn
7 Chung, M. S., Um, H. J., Kim, C. K. and Kim, G. H. (2007) Development of functional tea product using Circium japoinucum. Korean J. Food Culture 22: 261-265.
8 Wallace, S. N., Carrier, D. J. and Clausen, E. C. (2005) Batch solvent extraction of flavanolignans form milk thistle (Silybum marianum L. Gaertner). Phytochem. Anal. 16: 7-16   DOI   ScienceOn
9 Saller, R., Meier, R. and Brignoli, R. (2001) The use of silymarin in the treatment of liver diseases. Drugs 61, 2035- 2063.   DOI   ScienceOn
10 Lee, D. S., Kim, K.-S., Li, B., Choi, H.-G., Keo, S., Jun K.- Y., Park, J.-H. and Kim, Y.-C. (2012) Anti-inflammatory effects of the Cirsium japonicum var. ussuriense 70% ethanolic extract in RAW264.7 cells by heme oxygenase-1 expression. Kor. J. Pharmacogn. 43: 39-45.
11 Kim, S.-J., Kim, S.-Y., Kim, J.-A., Park, I. S., Yu, K.-Y., Chung, C.-H., Shim, J.-S., Jang, S.-I. and Jeong, S.-I. (2012) Inhibitory effect of Cirsium japonicum root or flower extract on hepatic stellate cells activation. Kor. J. Pharmacogn. 43: 27-31.
12 Otto, F. and Denis, W. (1912) On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12: 239-243.
13 Moreno, M. I. N., Isla, M. I. N., Sampietro, A. R. and Vattuone, M. A. (2000) Comparison of the free radical-scavenging activity of propolis from several region of Argentina. J. Ethnopharmacol. 71: 109-114.   DOI   ScienceOn
14 Cha, J. T., Cho, Y. S., Kim, I., Anno, T., Rahman, S. M. and Yanagita, T. (2001) Effect of hesperetin, a citrus flavonoid, on the liver triacylglycerol content and phosphatidate phosphohydrolase activity in orotic acid-fed rats. Pant. Foods Human Nutri. 56: 349-358.   DOI   ScienceOn
15 Vogel, G., Trost, W. and Braatz, R. (1975) Studies on the pharmacodynamics, including site and mode of action of silymarin: The antihepatotoxic principle from Silybum mar (L) Gaertn. Arzeiittelforsch 25: 2-9.
16 Gazák, R. D. and Walterova, Kren, V. (2007) Silybin and silymarin- new and emerging applications in medicine. Curr. Med. Chem. 14: 315-338.   DOI   ScienceOn
17 Liu, H., Du, Z. and Yuan, Q. (2009) A novel rapid method for simultaneous determination of eight active compounds in silymarin using reversed-phased UPLC-UV detector. J. Chromatogr. B. 877: 4159-4163.   DOI   ScienceOn
18 Jung, M. J., Heo, S.-I. and Wang, M.-H. (2008) Rat lens aldose reductase inhibitory of Taraxacum mongolicum and two Cirsium species. J. Appl. Biol. Chem. 51: 302-306.   DOI   ScienceOn
19 Bissell, D. M., Wang, S. S., Jargagin, W. R. and Roll, F. J. (1995) Cell-specific expression of transforming growth factor- beta in rat liver. Evidence for autocrine regulatin of hepatocyte proliferation. J. Clin. Invest. 96: 447-455.   DOI   ScienceOn
20 Gao, C. Gressner, G., Zoremba, M. and Gressner, A. M. (1996) Transforming growth factor beta (TGF-beta) expression in isolated and cultured rat hepatocytes. J. Cell Physiol. 167: 394-405.   DOI   ScienceOn
21 Liu, X., Hu, H. and Yin, J. Q. (2006) Therapeutic strategies against TGF-beta signaling pathway in hepatic fibrosis. Liver Int. 26: 8-22.   DOI   ScienceOn