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Inhibitory Effect of Elastase and Tyrosinase of Ginsenoside $F_1$ Isolated from Panax ginseng Leaves  

Hong, Se Chul (International Ginseng & Herb Research Institute)
Yoo, Nam Hee (International Ginseng & Herb Research Institute)
Yoo, Ji Hyun (International Ginseng & Herb Research Institute)
Lee, Kun Hee (International Ginseng & Herb Research Institute)
Kim, Bo Ram (International Ginseng & Herb Research Institute)
Lee, Ho Joo (International Ginseng & Herb Research Institute)
Kim, Jong Min (International Ginseng & Herb Research Institute)
Seong, Nak Sul (International Ginseng & Herb Research Institute)
Pyo, Mi Kyung (International Ginseng & Herb Research Institute)
Publication Information
Korean Journal of Pharmacognosy / v.44, no.1, 2013 , pp. 10-15 More about this Journal
Abstract
This study was carried to establish a simple isolation and purification method of ginsenoside $F_1$ from leaves of Panax ginseng and was to evaluate the inhibitory effect of purified ginsenoside $F_1$ on the activities of elastase and tyrosinase. The content of ginsenoside $F_1$ was 90-fold higher in leaves than in root of ginseng. Ginsenoside $F_1$ was isolated from EtOAc fraction between EtOAc and alkalized water of 80% EtOH extract after remove of hydrophobic components. The 50% inhibitory concentration ($IC_{50}$) of ginsenoside $F_1$ on elastase activity and tyrosinase activity was 1.07 mM and 1.81 mM, respectively. Especially, inhibitory effect of ginsenoside $F_1$ on tyrosinase activity was higher than that of arbutin ($IC_{50}$; 2.20 mM). These results indicate that ginsenoside $F_1$ have a potential for industrial cosmetic materials.
Keywords
Panax ginseng; Leaves; Ginsenoside $F_1$; Elastase; Tyrosinase;
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