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Comparative Study on Biological Activities and Ingredient Contents of Different Solvent Extracts of Samchulkunbi-tang  

Jin, Seong Eun (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Seo, Chang-Seob (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Ha, Hyekyung (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Lim, Hye-Sun (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Kim, Yeji (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Jeon, Woo-Young (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Yoo, Sae-Rom (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Kim, Jung-Hoon (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Shin, In-Sik (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Kim, Seong-Sil (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Shin, Na Ra (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Lee, Mee-Young (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Jeong, Soo-Jin (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Kim, Ohn Soon (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Shin, Hyeun-Kyoo (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Publication Information
Korean Journal of Pharmacognosy / v.43, no.4, 2012 , pp. 328-337 More about this Journal
Abstract
In order to investigate anti-inflammatory, anti-allergic and anti-obesity activities of Samchulkunbi-tang (SCT; Shen zhu jian pi-tang) water and 70% ethanol (EtOH) extracts, in vitro inhibitory activities against nitric oxide (NO), prostaglandin $E_2$ $PGE_2$), interleukin (IL)-6 and tumor necrosis factor (TNF)-${\alpha}$ production in lipopolysaccharide-stimulated RAW 264.7 cells, and macrophage-derived chemokine (MDC/CCL22) and regulated on activation of normal T-cell-expressed and -secreted (RANTES/CCL5) production in TNF-${\alpha}$/interferon-${\gamma}$-stimulated HaCaT and BEAS-2B cells as well as glycerol-3-phosphate dehydrogenase (GPDH) activity and leptin production in 3T3-L1 cells were determined. A HPLC was used for quantification of the seven marker components (albiflorin, paeoniflorin, liquiritin, naringin, hesperidin, poncirin and glycyrrhizin) of SCT water and 70% EtOH extracts. SCT showed inhibitory effects against MDC and RANTES production in HaCaT cells, as well as RANTES production in BEAS-2B cells. In addition, SCT reduced not only NO, $PGE_2$, IL-6 and TNF-${\alpha}$ production in RAW 264.7 cells, but also GPDH activity and leptin production in 3T3-L1 cells. Furthermore, the biological activities and the contents of six compounds (except paeoniflorin) were higher in 70% EtOH extract than water extract. These results suggest that SCT has anti-inflammatory, anti-allergic and anti-obesity activities. These efficacies of 70% EtOH extract are relatively higher than that of water extract.
Keywords
Samchulkunbi-tang; Different solvent extract; Anti-inflammation; Anti-allergy; Anti-obesity;
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