Browse > Article

Inhibitory Effect of Artemisinic Acid Isolated from Artemisia Annua L on the MDC in HaCaT Keratinocytes  

Kang, Gyeoung-Jin (Department of Pharmacology, School of Medicine, Jeju National University)
Kang, Na-Jin (Department of Pharmacology, School of Medicine, Jeju National University)
Han, Sang-Chul (Department of Pharmacology, School of Medicine, Jeju National University)
Koo, Dong-Hwan (Department of Pharmacology, School of Medicine, Jeju National University)
Kim, Young-Soo (Biospectrum Life Science Institute)
Lee, Jin-Hyuck (Biospectrum Life Science Institute)
Kim, Sang-Chul (Biospectrum Life Science Institute)
Park, Deok-Hoon (Biospectrum Life Science Institute)
Lee, Jong-Sung (Department of Dermatological Health Management, Eul-Ji University)
Kang, Hee-Kyung (Department of Pharmacology, School of Medicine, Jeju National University)
Yoo, Eun-Sook (Department of Pharmacology, School of Medicine, Jeju National University)
Publication Information
Korean Journal of Pharmacognosy / v.43, no.3, 2012 , pp. 217-223 More about this Journal
Abstract
In the present study, we investigated anti-inflammatory activity of artemisinic acid in HaCaT cells and RAW264.7 cells. Artemisinic acid showed inhibitory activity on macrophage-derived chemokines (MDC) expression, a factor related with atopic dermatitis (AD), in interferon (IFN)-${\gamma}$ and tumor necrosis factor (TNF)-${\alpha}$-stimulated HaCaT cells. In the study on action mechanism, pretreated artemisinic acid reduced the phosphorylation of STAT1 and p38 and the degradation of $I{\kappa}B$ by IFN-${\gamma}$ and TNF-${\alpha}$ stimulations. However, artemisinic acid didn't show the inhibitory activity on LPS-induced inflammatory mediators (NO, $PGE_2$, IL-6) in RAW264.7 cell. These results indicate that artemisinic acid inhibits IFN-${\gamma}$ and TNF-${\alpha}$-induced MDC expression through inhibition of signal factors, STAT1, NF-${\kappa}B$, and p38, in HaCaT keratinocytes.
Keywords
Artemisinic acid; Inflammation; MDC/CCL22; HaCaT keratinocyte; RAW264.7; Macrophages;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Coleman, J. W. (2001) Nitric oxide in immunity and inflammation. Int. Immunopharmacol. 1: 1397-1406.   DOI   ScienceOn
2 Tripathi, P., Tripathi, P., Kashyap, L. and Singh, V. (2007) The role of nitric oxide in inflammatory reactions. FEMS Immunol. Med. Microbiol. 51: 443-452.   DOI
3 Abramovits, W. (2005) Atopic dermatitis. J. Am. Acad. Dermatol. 53: S86-93.   DOI   ScienceOn
4 Bonness, S. and Bieber, T. (2007) Molecular basis of atopic dermatitis. Curr. Opin. Allergy Clin. Immunol. 7: 382-386.   DOI   ScienceOn
5 Pease, J. E. and Williams, T. J. (2006) Chemokines and their receptors in allergic disease. J. Allergy Clin. Immunol. 118: 305-18.   DOI   ScienceOn
6 Imai, T., Nagira, M., Takagi, S., Kakizaki, M., Nishimura, M., Wang, J., Gray, P. W., Matsushima, K. and Yoshie, O. (1999) Selective recruitment of CCR4-bearing Th2 cells toward antigen-presenting cells by the CC chemokines thymus and activation-regulated chemokine and macrophage-derived chemokine. Int. Immunol. 11: 81-88.   DOI   ScienceOn
7 Kakinuma, T., Nakamura, K., Wakugawa, M., Mitsui, H., Tada, Y., Saeki, H., Torii, H., Komine, M., Asahina, A. and Tamaki, K. (2002) Serum macrophage-derived chemokine (MDC) levels are closely related with the disease activity of atopic dermatitis. Clin. Exp. Immunol. 127: 270-273.   DOI   ScienceOn
8 Leung, T. F., Ma, K. C., Hon, K. L., Lam, C. W., Wan, H., Li, C. Y. and Chan, I. H. (2003) Serum concentration of macrophage- derived chemokine may be a useful inflammatory marker for assessing severity of atopic dermatitis in infants and young children. Pediatr. Allergy Immunol. 14: 296-301.   DOI   ScienceOn
9 Hijnen, D., De Bruin-Weller, M., Oosting, B., Lebre, C., De Jong, E., Bruijnzeel-Koomen, C. and Knol, E. (2004) Serum thymus and activation-regulated chemokine (TARC) and cutaneous T cell-attracting chemokine (CTACK) levels in allergic diseases: TARC and CTACK are disease-specific markers for atopic dermatitis. J. Allergy Clin. Immunol. 113: 334-340.   DOI   ScienceOn
10 Aktan, F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75: 639-653.   DOI   ScienceOn
11 Flower, R. J. (2006) Prostaglandins, bioassay and inflammation. Br. J. Pharmacol. 147: S182-92.
12 Ricciotti, E. and FitzGerald, G. A. (2011) Prostaglandins and inflammation. Arterioscler. Thromb. Vasc. Biol. 31: 986-1000.   DOI   ScienceOn
13 Maruotti, N., Cantatore, F. P., Crivellato, E., Vacca, A. and Ribatti, D. (2007) Macrophages in rheumatoid arthritis. Histol. Histopathol. 22: 581-586.
14 Pivarcsi, A., Nagy, I. and Kemeny, L. (2005) Innate Immunity in the Skin: How Keratinocytes Fight Against Pathogens. Curr. Immunol. Rev. 1: 29-42.   DOI
15 Homey, B., Steinhoff, M., Ruzicka, T. and Leung, D. Y. (2006) Cytokines and chemokines orchestrate atopic skin inflammation. J. Allergy Clin Immunol. 118: 178-89.   DOI   ScienceOn
16 Bhakuni, R. S., Jain, D. C., Sharma, R. P. and Kumar, S. (2001) Secondary metabolites of Artemisia annua and their biological activity. Curr. Sci. 80: 35-48.
17 Xiao, T., Kagami, S., Saeki, H., Sugaya, M., Kakinuma, T., Fujita, H., Yano, S., Mitsui, H., Torii, H., Komine, M., Asahina, A., Nakamura, K. and Tamaki, K. (2003) Both IL-4 and IL-13 inhibit the TNF-alpha and IFN-gamma enhanced MDC production in a human keratinocyte cell line, HaCaT cells. J. Dermatol. Sci. 31(2): 111-7.   DOI   ScienceOn
18 Iqbal, S., Younas, U., Chan, K. W., Zia-Ul-Haq, M. and Ismail, M. (2012) Chemical composition of artemisia annua L. leaves and antioxidant potential of extracts as a function of extraction solvents. Molecules 17: 6020-6032.   DOI   ScienceOn
19 Lee, J., Kim, M. H., Lee, J. H., Jung, E., Yoo, E. S. and Park, D. (2012) Artemisinic acid is a regulator of adipocyte differentiation and C/EBP delta expression. J. Cell. Biochem. 113: 2488-2499.   DOI   ScienceOn
20 Ngamwongsatit, P., Banada, P. P., Panbangred, W. and Bhunia, A. K. (2008) WST-1-based cell cytotoxicity assay as a substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line. J. Microbiol. Methods 73: 211-215.   DOI   ScienceOn
21 Kang, G. J., Lee, H. J., Yoon, W. J., Yang E. J., Park, S. S., Kang H. K., Park, M. H. and Yoo, E. S. (2008) Prunus Yedoensis Inhibits the Inflammatory Chemokines, MDC and TARC, by Regulating the STAT1-Signaling Pathway in IFN-${\gamma}$-stimulated HaCaT Human Keratinocytes. Biomol. Ther. 16: 394-402.   DOI   ScienceOn
22 Lee, H. J., Oh, T. H., Yoon, W. J., Kang, G. J., Yang E. J., Park, S. S., Lee, N. H., Kang H. K. and Yoo, E. S. (2008) Eutigoside C inhibits the production of inflammatory mediators (NO, PGE2, IL-6) by down-regulating ${\kappa}B$ and MAP kinase activity in LPS-stimulated RAW264.7 cells. J. Pharm. Pharmacol. 60: 917-924.   DOI   ScienceOn
23 Horikawa, T., Nakayama, T., Hikita, I., Yamada, H., Fujisawa, R., Bito, T., Harada, S., Fukunaga, A., Chantry, D., Gray, P. W., Morita, A., Suzuki, R., Tezuka, T., Ichihashi, M. and Yoshie, O. (2002) IFN-gamma-inducible expression of thymus and activation-regulated chemokine/CCL17 and macrophage- derived chemokine/CCL22 in epidermal keratinocytes and their roles in atopic dermatitis. Int. Immunol. 14: 767-773.   DOI   ScienceOn
24 Pivarcsi, A. and Homey, B. (2005) Chemokine networks in atopic dermatitis: traffic signals of disease. Curr. Allergy Asthma Rep. 5: 284-290.   DOI   ScienceOn
25 Jeong, S. I., Choi, B. M. and Jang, S. I. (2010) Sulforaphane suppresses TARC/CCL17 and MDC/CCL22 expression through heme oxygenase-1 and NF-kappaB in human keratinocytes. Arch. Pharm. Res. 33: 1867-1876.   DOI   ScienceOn
26 Yamashita, U. and Kuroda, E. (2002) Regulation of macrophage- derived chemokine (MDC, CCL22) production. Crit. Rev. Immunol. 22: 105-114.
27 Gough, D. J., Levy, D. E., Johnstone, R. W. and Clarke, C. J. (2008) IFNgamma signaling-does it mean JAK-STAT? Cytokine Growth Factor Rev. 19: 383-394.   DOI   ScienceOn
28 Hongqin, T., Xinyu, L., Heng, G., Lanfang, X., Yongfang, W. and Shasha, S. (2011) Triptolide Inhibits IFN-gamma Signaling via the Jak/STAT Pathway in HaCaT Keratinocytes. Phytother. Res. 25(11): 1678-1685
29 Ju, S. M., Song, H. Y., Lee, S. J., Seo, W. Y., Sin, D. H., Goh, A. R., Kang, Y. H., Kang, I. J., Won, M. H., Yi, J. S., Kwon, D. J., Bae, Y. S., Choi, S. Y. and Park, J. (2009) Suppression of thymus- and activation-regulated chemokine (TARC/ CCL17) production by 1,2,3,4,6-penta-O-galloyl-beta-D-glucose via blockade of NF-kappaB and STAT1 activation in the HaCaT cells. Biochem. Biophys. Res. Commun. 387: 115-120.   DOI   ScienceOn
30 Himaya, S. W., Ryu, B., Qian, Z. J. and Kim, S. K. (2010) Sea cucumber, Stichopus japonicus ethyl acetate fraction modulates the lipopolysaccharide induced iNOS and COX-2 via MAPK signaling pathway in murine macrophages. Environ. Toxicol. Pharmacol. 30: 68-75.   DOI   ScienceOn
31 Kim, H. J., Tsoyi, K., Heo, J. M., Kang, Y. J., Park, M. K., Lee, Y. S., Lee, J. H., Seo, H. G., Yun-Choi, H. S. and Chang, K. C. (2007) Regulation of lipopolysaccharide-induced inducible nitric-oxide synthase expression through the nuclear factor-kappaB pathway and interferon-beta/tyrosine kinase 2/Janus tyrosine kinase 2-signal transducer and activator of transcription-1 signaling cascades by 2-naphthylethyl- 6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline (THI 53), a new synthetic isoquinoline alkaloid. J. Pharmacol. Exp. Ther. 320: 782-789.