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Nitric Oxide Production Inhibitory Effect and Antibacterial Activity of the Extract and Fractions from Paeoniae Radix  

Im, Do-Youn (Dept. of Beauty Science, Kwangju Women's University)
Lee, Kyoung-In (Biotechnology Industrialization Center, Dongshin University)
Publication Information
Korean Journal of Pharmacognosy / v.43, no.2, 2012 , pp. 173-178 More about this Journal
Abstract
In this study, we investigated antibacterial activity and nitric oxide production inhibitory effect of the methanol extract and its fractions from Paeoniae Radix. In antibacterial activity by the disc diffusion assay against S. aureus, S. epidermidis and P. aeruginosa, the ethyl acetate fraction showed stronger antibacterial activity than other fractions and the extract. Moreover, the ethyl acetate fraction showed strong nitric oxide (NO) production inhibitory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 cell. However, in NO scavenging ability, the chloroform fraction was higher than the other fractions and the extract. In the lactate dehydrogenase (LDH) assay against RAW 264.7 cell, the extract and fractions were exhibited normal LDH release level as nontoxic result without the ehtyl acetate fraction of 100 ${\mu}g/ml$. As a result, the ethyl acetate fraction and chloroform fraction of the methanol extract from Paeoniae Radix could be applicable to functional materials for antibacterial and anti-inflammatory related fields, respectively.
Keywords
Paeoniae radix; Antibacterial; Anti-inflammatory; Nitric oxide; Cytotoxicity;
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Times Cited By KSCI : 13  (Citation Analysis)
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