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Changes of Antioxidant Activity and Ingredient in Medicinal Plants by Processing  

Cha, Bae-Cheon (Department of Pharmaceutical Engineering, College of Health Sciences, Sangji University)
Jun, Kyung-Su (Department of Pharmaceutical Engineering, College of Health Sciences, Sangji University)
Publication Information
Korean Journal of Pharmacognosy / v.43, no.1, 2012 , pp. 46-53 More about this Journal
Abstract
This study was conducted to investigate the change of antioxidant activity and ingredient in 4 species medicinal plant by processing. As a result, EtOAc extract and n-BuOH extract obtained from Cistanche deserticola Y. C. Ma and processed Cistanche deserticola Y. C. Ma showed difference on the experiment of antioxidant activity by DPPH method. Change of ingredient also was confirmed by TLC and HPLC analyses. Two main compounds of antioxidant activity change were isolated by column chromatography from the EtOAc extract and n-BuOH extract of Cistanche deserticola Y. C. Ma. The chemical structure of the compound 1 isolated from EtOAc extract of Cistanche deserticola Y. C. Ma was elucidated as 2'-acetylacteoside by means of IR and NMR analyses. Also, the chemical structure of the compound 2 isolated from n-BuOH extract of Cistanche deserticola Y. C. Ma was confirmed as acteoside. The antioxidant activity of processed Cistanche deserticola Y. C. Ma. was declined by 3 time decrements of 2'-acetylacteoside such as 1.89 mg/g to 0.62 mg/g and 2 time decrements of acteoside such as 5.81 mg/g to 2.67 mg/g by processing.
Keywords
Processing; Change; Antioxidant activity; DPPH; HPLC; Cistanche deserticola; 2'-Acetylacteoside; Acteoside;
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