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Screening on Allogeneic Mixed Lymphocyte Culture Inhibitory Activity for the Extracts of Marine-Derived Microorganisms  

Yun, Keum-Ja (Department of Chemistry, Pukyong National University)
Oh, Keun-Hee (Department of Biomedical Sciences/Transplantation Research Institute, Seoul National University College of Medicine)
Lee, Dong-Sup (Department of Biomedical Sciences/Transplantation Research Institute, Seoul National University College of Medicine)
Choi, Hong-Dae (Department of Chemistry, Dongeui University)
Kang, Jung-Sook (School of Dentistry, Pusan National University)
Son, Byeng-Wha (Department of Chemistry, Pukyong National University)
Publication Information
Korean Journal of Pharmacognosy / v.42, no.4, 2011 , pp. 354-360 More about this Journal
Abstract
In order to screen new allogeneic mixed lymphocyte culture (allo-MLR) inhibitor which is expected to be immunomodulating drug lead, we have investigated allo-MLR inhibitory activity on the marine-derived symbiotic microorganisms (1,895 strains) from the marine algae. The potent inhibitory activities (over 45% inhibition of proliferation at 10 and 2 ${\mu}g/ml$) without cytotoxicity were observed in the extracts of 46 strains. While, the significant stimulating activities (over 100% proliferation at 10 and 2 ${\mu}g/ml$) without cytotoxicity were observed in the extracts of 5 strains. In the second assay using 46 bioactive strains, 14 strains exhibited again significant allo-MLR inhibitory activity. Finally, 11 strains among the 14 strains inhibited proliferation and IFN-${\gamma}$ production of CD4+ T cells during the stimulation with specific antigen in the third assay. On the basis of above results, the marine algae is nice source for isolation of immunomodulating microorganism, and the marine algae-associated microorganism is also nice target for development of the new immunomodulating drug lead.
Keywords
Marine algae; Marine-derived microorganism; Allogeneic mixed lymphocyte culture (allo-MLR) inhibitor; Immunomodulation;
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Times Cited By KSCI : 4  (Citation Analysis)
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