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The Effects of Dictamni Radicis Cortex on the iNOS Expression and Proinflammatory Cytokines Production  

Park, Jeong-Suk (Department of Alternative Medicine Nambu University)
Shin, Tae-Yong (College of Pharmacy, Woosuk University)
Kim, Dae-Keun (College of Pharmacy, Woosuk University)
Lee, Jae-Hyeok (Department of Oriental Pharmaceutical Development, Nambu University)
Publication Information
Korean Journal of Pharmacognosy / v.42, no.4, 2011 , pp. 348-353 More about this Journal
Abstract
The aim of the present study is to investigate the cytokine production inhibitory effect of a Dictamni Radicis Cortex (DRC). DRC has been commonly used as important medicinal herb in China and it used to control eczema, atopic dermatitis, fever and inflammatory diseases. Inflammation, such as a bacterial infection in vivo metabolites, such as external stimuli or internal stimuli to the defense mechanisms of the biological tissue a variety of intracellular regulatory factors deulin inflammatory TNF-${\alpha}$, IL-$1{\beta}$, IL-6, IL-8, such as proinflammatory cytokines, prostagrandin, lysosomal enzyme, free radicals are involved in a variety of mediators. The present study was designed to determine the effect of the DRC on proinflammatory factors such as NO, iNOS expression and TNF-${\alpha}$, IL-$1{\beta}$, IL-6 in lipopolysaccharide (LPS) - stimulated RAW264.7 cells. The cell toxicity was determined by MTS assay. To evaluate of anti-inflammatory effect of DRC, amount of NO was measured using the NO detection kit and the iNOS expression was measured by reverse transcriptase polymerase chain reaction (RT-PCR). And proinflammatory cytokines were measured by ELISA kit. As a result, the DRC reduced NO, iNOS expression and TNF-${\alpha}$, IL-$1{\beta}$, IL-6 production without cytotoxicity. Our results suggest that the DRC may have an anti-inflammatory property through suppressing inflammatory mediator productions.
Keywords
Dictamni Radicis Cortex; Cytokine; NO; iNOS; TNF-${\alpha}$;
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