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Constituents of Flowers of Carthamus tinctorius L. and Their Antioxidant Activity  

Choi, Hyun-Gyu (College of Pharmacy, Yeungnam University)
Jiang, Yan-Fu (College of Pharmacy, Yeungnam University)
Park, Sung-Hee (Department of Health & Medical Administration, Gyeongnam Provincial Geochangcollege)
Son, Ae-Ryang (Department of Beauty Design, Gyeongnam Provincial Geochangcollege)
Na, Min-Kyun (College of Pharmacy, Yeungnam University)
Lee, Seung-Ho (College of Pharmacy, Yeungnam University)
Publication Information
Korean Journal of Pharmacognosy / v.42, no.2, 2011 , pp. 110-116 More about this Journal
Abstract
As part of our ongoing study focused on the discovery of antioxidants from natural products by measuring the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity, methanol extract of flowers of Carthamus tinctorius L. was found to show potent antioxidant activity. Activity-guided fractionation of the methanol extract lead to the isolation of twenty compounds including two flavonol glycosides, quercertin-3-O-${\beta}$-D-glucopyranoside (12) and kaempferol-3-O-${\alpha}$-L-rhamnopyranosyl-${\beta}$-D-glucopyranoside (18), two flavanone glycosides, (2S)-4',5,6,7-tetrahydroxyflavanone 6-O-${\beta}$-D-glucopyranoside (15) and (2R)-5,7,8',4-tetrahydroxyflavanone 8-O-${\beta}$-D-glucopyranoside (16), and two acetylenic glycosides, 8Z-decaene-4,6-diyne-1-O-${\beta}$-D-glucopyranoside (13) and 4,6-decadiyne-1-O-${\beta}$-D-glucopyranoside (14). Their chemical structures were identified by using spectroscopic analysis. Among them, compounds 12-18 were tested in DPPH assay. Compounds 13-16 were first reported to their antioxidant activity. Quercertin-3-O-${\beta}$-D-glucopyranoside (12) showed the most potent inhibitory effect on DPPH with $IC_{50}$ value of 56.7 ${\mu}M$.
Keywords
Carthamus tinctorius; DPPH; Quercertin-3-O-${\beta}$-D-glucopyranoside; Acetylenic glycoside;
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