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Ameliorating Effects of Herbal Ethanol Extract from Gynostemma pentaphyllum on Chronic Stress-Induced Anxiety in Mice  

Choi, Hyun-Sook (College of Pharmacy, Chungbuk National University)
Shin, Kun-Seong (College of Pharmacy, Chungbuk National University)
Choi, Soon-Ok (College of Pharmacy, Chungbuk National University)
Kim, Seung-Hwan (College of Physical Education, Kyunghee University)
Hwang, Bang-Yeon (College of Pharmacy, Chungbuk National University)
Lee, Chong-Kil (College of Pharmacy, Chungbuk National University)
Lee, Myung-Koo (College of Pharmacy, Chungbuk National University)
Publication Information
Korean Journal of Pharmacognosy / v.42, no.1, 2011 , pp. 32-37 More about this Journal
Abstract
The effects of herbal ethanol extract from Gynostemma pentaphyllum (GP extract) on chronic stress-induced anxiety in mice were investigated. The animals were treated with GP extracts (50 and 100 mg/kg/day, p.o.) for 21 days before exposure to electric footshock (EF; duration and interval 10 sec for 3 min, 2 mA) for chronic stress once a day. The ambulatory locomotor activity was reduced by chronic EF stress and it was recovered by 12.9-15.1% in GP extract-treated groups. The grip strength was also significantly decreased by chronic EF stress, however, the EF-stressed groups treated with GP extract increased grip strength from 13.9% to 56.8% compared to EF-stressed groups. In addition, the serum levels of corticosterone were significantly elevated by chronic EF stress to 197% of the control levels, which was reduced to 73.1% by treatment with GP extract (100 mg/kg). In contrast, the brain levels of dopamine and serotonin were reduced to 67.6% and 63.1% by chronic EF stress, which was recovered to 90% of the control levels by treatment with GP extract. These results indicate that GP extract shows the ameliorating effects on chronic EF stress-induced anxiety in mice and it can be developed as the promising anti-anxiety agent.
Keywords
Gynostemma pentaphyllum; Chronic stress-induced anxiety; Ambulatory locomotor activity; Grip strength; Corticosterone; Dopamine; Serotonin;
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