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The Immunoadjuvant Activity of The Water-Extract of Korean mistletoe (Viscum album var. coloratum) Fruit  

Lee, Jung-Lim (Department of Microbiology, College of medicine, Konyang University)
Ahn, Jae-Hyung (Department of Microbiology, College of medicine, Konyang University)
Hwang, Seong-Gu (Division of Animal Life and Environmental Science, Hankyong National University)
Jung, Yeon-Hwa (Department of Microbiology, College of medicine, Konyang University)
Yang, Hyo-Seon (Department of Microbiology, College of medicine, Konyang University)
Kang, Tae-Bong (Department of Biotechnology & Food Science, Handong University)
Kim, Jong-Bae (Department of Biotechnology & Food Science, Handong University)
Yoo, Yung-Choon (Department of Microbiology, College of medicine, Konyang University)
Publication Information
Korean Journal of Pharmacognosy / v.41, no.4, 2010 , pp. 275-281 More about this Journal
Abstract
To evaluate the immunomodulatory activity of a water extract (KMF-WE) of Korean mistletoe (Viscum album var. coloratum) fruit, we examined its ability to induce humoral and cellular immune response against keyhole limpet hemocyanine (KLH). Immunized mice with KLH admixed with KMF-WE (KLH/KMF-WE) showed significant induction of KLH-specific antibodies compared to mice immunized with KLH alone. The assay for determining isotypes of antibodies revealed that KMFWE augmented KLH-specific-IgG1 and -IgG2a production. In vitro T lymphocyte proliferation analysis against KLH revealed that the splenocytes of mice immunized with KLH/KMF-WE showed a significantly higher proliferative ability than those from mice immunized with KLH alone. The culture supernatants of splenocytes, which were harvested from mice immunized with KLH/KMF-WE, showed higher levels of both Th-1 type (IL-2, IFN-${\gamma}$) and Th-2 type (IL-4) cytokines in response to KLH stimulation compared to those from mice immunized with KLH alone. Also, in delayed-type hypersensitivity (DTH) assay, mice immunized with KLH/KMF-WE showed a significantly higher reaction to KLH than mice treated with KLH alone. These results suggest that KMF-WE possess immunoadjuvant activity to enhance both antigen-specific humoral and cellular immune responses against protein antigens (KLH).
Keywords
Korean mistletoe; fruit-water extract; adjuvant; antibody; cytokines;
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