Browse > Article

Effect of Eriobotrya japonica Leaf and Seed Extracts on Adipogenesis  

Min, Oh-Jin (Dept. of Medicinal Plant Resources, Mokpo National University)
Oh, Jin (Dept. of Medicinal Plant Resources, Mokpo National University)
Kim, Hyeon-A (Dept. of Food & Nutrition, Mokpo National University)
Kim, Min-Sook (Dept. of Food & Nutrition, Mokpo National University)
Baek, Hum-Young (INSPharm)
Kim, Yong-Jae (INSPharm)
Rhyu, Dong-Young (Dept. of Medicinal Plant Resources, Mokpo National University)
Publication Information
Korean Journal of Pharmacognosy / v.41, no.4, 2010 , pp. 270-274 More about this Journal
Abstract
Obesity is caused from an imbalance between energy intake and expenditure, which may lead to pathologic growth of adipocytes and accumulation of fat in tissue. We examined the inhibitory effects of Eriobotrya japonica leaf and seed extracts on lipid absorption in vitro and fat accumulation during the differentiation of 3T3-L1 to adipocytes. 3T3-L1 preadipocytes were stimulated with DMEM media containing 10% FBS, 0.5 mM 3-isobuthyl-1-methyxanthine (IBMX), $5\;{\mu}g/ml$ insulin, and $1\;{\mu}g/ml$ dexamethasone for differentiation to adipocytes. E. japonica leaf extract at concentration of 0.5 or 1 mg/ml inhibited pancreatic lipase activity. The cell viability of 3T3-L1 adipocytes slightly reduced about 3% by treatment of E. Japonica leaf and seed extracts. The leaf and seed extracts of E. japonica effectively inhibited the accumulations of lipid droplet and expression of $C/EBP{\alpha}$ promoting adipogenesis. Thus, this data suggest that E. japonica leaf and seed extracts inhibit fat accumulation through regulation of $C/EBP{\alpha}$, and leaf extract is more effective in lipid absorption and adipogenesis than seed extract.
Keywords
Eriobotrya japonica; 3T3-L1 adipocytes; peroxisome proliferator-activated receptor(PPAR) ${\gamma}$; CCAAT/enhancer-binding protein(C/EBP) ${\alpha}$; adipogenesis;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Chen, J., Li, W. L., Wu, J. L., Ren, B. R. and Zhang, H. Q. (2008) Hypoglycemic effects of a sesquiterpene glycoside isolated from leaves of loquat (Eriobotrya japonica (Thunb.) Lindl.). Phytomedicine. 15(1-2): 98-102.   DOI   ScienceOn
2 Lee, H., Kang, R. and Yoon, Y. (2010) Shikonin inhibits fat accumulation in 3T3-L1 adipocytes. Phytother. Res. 24(3): 344-351.   DOI   ScienceOn
3 이경인, 김선민 (2009) 비파 잎 추출물의 항산화 및 항균 활성. 한국식품영양과학회지 38(3): 267-273.   과학기술학회마을   DOI   ScienceOn
4 배남규, 권인선, 조영채 (2009) 한국인의 10년간 비만수준의 변화 양상: 1997-2007. 대한비만학회지 18(1): 24-30.
5 Spiegelman, B. M. and Flier, J. S. (2001) Obesity and the regulation of energy balance. Cell 104(4): 531-543.   DOI   ScienceOn
6 Kubota, H., Morii, R., Kojima-Yuasa, A., Huang, X., Yano, Y. and Matsui-Yuasa, I. (2009) Effect of Zizyphus jujuba extract on the inhibition of adipogenesis in 3T3-L1 preadipocytes. Am. J. Chin. Med. 37(3): 597-608.   DOI   ScienceOn
7 김태훈, 신승렬, 김태완, 이인철, 박문영, 조철훈 (2009) 비파씨로부터 Tyrosinase 저해 활성물질의 분리. 한국식품저장유통학회지. 16(3): 435-441.   과학기술학회마을
8 김은, 김민숙, 류동영, 민오진, 백흠영, 김용재, 김현아 (2009) db/db 마우스에서 비파의 혈당 저하 효과. 한국식품영양학회지. 22(2): 159-165.   과학기술학회마을
9 Nugent, C., Prins, J. B., Whitehead, J. P., Savage, D., Went-worth, J. M., Chatterjee, V. K. and O'Rahilly, S. (2001) Potentiation of glucose uptake in 3T3-L1 adipocytes by PPAR gamma agonists is maintained in cells expressing a PPAR gamma dominant-negative mutant: evidence for selectivity in the downstream responses to PPAR gamma activation. Mol. Endocrinol. 15(10): 1729-1738.   DOI   ScienceOn
10 Zong, W. and Zhao, G. (2007) Corosolic acid isolation from the leaves of Eriobotrta japonica showing the effects on carbohydrate metabolism and differentiation of 3T3-L1 adipoadipocytes. Asia Pac. J. Clin. Nutr. 16(Suppl 1): 346-352.
11 김민수, 김보연, 박찬선, 윤병대, 안순철, 오원근, 안종석 (2006) 백자인 추출물에 의한 pancreatic lipase의 저해 효과. 생명과학회지. 16(2): 328-332.   과학기술학회마을
12 Li, M. and Cheung, B. M. (2009) Pharmacotherapy for obesity. Br. J. Clin. Pharmacol. 68(6): 804-810.   DOI   ScienceOn
13 Nakai, M., Fukui, Y., Asami, S., Toyoda-Ono, Y., Iwashita, T., Shibata, H., Mitsunaga, T., Hashimoto, F. and Kiso, Y. (2005) Inhibitory effects of oolong tea polyphenols on pancreatic lipase in vitro. J. Agric. Food Chem. 53(11): 4593-4598.   DOI   ScienceOn
14 Yang, J. Y., Della-Fera, M. A., Rayalam, S., Ambati, S., Hartzell, D. L., Park, H. J. and Baile, C. A. (2008) Enhanced inhibition of adipogenesis and induction of apoptosis in 3T3-L1 adipocytes with combinations of resveratrol and quercetin. Life Sci. 82(19-20): 1032-1039.   DOI   ScienceOn
15 Lee, S. H., Kim, K. Y., Ryu, S. Y., Yoon, Y., Hahm, D. H., Kang, S. A., Cho, S. H., Lim, J. S., Moon, E. Y., Yoon, S. R., Lee, H. G., Yoon, D. Y. and Yang, Y. (2010) Asarone inhibits adipogenesis and stimulates lipolysis in 3T3-L1 adipocytes. Cell Mol. Biol. 56(Suppl): 1215-1222.
16 Hu, C., Chen, L., Xin, Y. and Cai, Q. (2006) Determination of corosolic acid in Eriobotrya japonica leaves by reversedphase high performance liquid chromatography. Se. Pu. (Chinese Journal of Chromatography) 24(5): 492-494.
17 Huang, Y., Li, J., Meng, X. M., Jiang, G. L., Li, H., Cao, Q., Yu, S. C., Lv, X. W. and Cheng, W. M. (2009) Effect of triterpene acids of Eriobotrya japonica (Thunb.) Lindl. leaf and MAPK signal transduction pathway on inducible nitric oxide synthase expression in alveolar macrophage of chronic bron-chitis rats. Am. J. Chin. Med. 37(6): 1099-1111.   DOI   ScienceOn
18 김호철 (2001) 한약약리학. 도서출판 집문당, p370-371.
19 서부일, 최호영 (2004) 임상한방본초학. 도서출판 영림사, p676-677.
20 Takuma, D., Guangchen, S., Yokota, J., Hamada, A., Onogawa, M., Yoshioka, S., Kusunose, M., Miyamura, M., Kyotani, S. and Nishioka, Y. (2008) Effect of Eriobotrya japonica seed extract on 5-fluorouracil-induced mucositis in hamsters. Biol. Pharm. Bull. 31(2): 250-254.   DOI   ScienceOn
21 Sun, G., Zhang, Y., Takuma, D., Onogawa, M., Yokota, J., Hamada, A., Yoshioka, S., Kusunose, M., Miyamura, M., Kyotani, S. and Nishioka, Y. (2007) Effect of orally administered Eriobotrya japonica seed extract on allergic contact dermatitis in rats. J. Pharm. Pharmacol. 59(10): 1405-1412.   DOI   ScienceOn
22 Kim, S. H. and Shin, T. Y. (2009) Anti-inflammatory effect of leaves of Eriobotrya japonica correlating with attenuation of p38 MAPK, ERK, and NF-kappaB activation in mast cells. Toxicol. In Vitro. 23(7): 1215-1219.   DOI   ScienceOn
23 박용서, 박윤점, 김현주, 임명희, 이미경, 김영민, 조자용, 허북구 (2008) 비파 부위별 에탄올 추출물의 생리활성. 원예과학기술지 26(1): 75-80.
24 Payne, V. A., Au, W. S., Lowe, C. E., Rahman, S. M., Friedman, J. E., O'Rahilly, S. and Rochford, J. J. (2009) C/EBP transcription factors regulate SREBP1c gene expression during adipogenesis. Biochem. J. 425(1): 215-223.
25 강재헌 (2008) 비만치료의 최신지견-약물요법을 중심으로. 가정의학회지 29(4): S156-S157.