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The Immunostimulatory Activity of The Water-Extract of Korean Mistletoe Fruit to Activate Murine Peritoneal Macrophages  

Lee, Jung-Lim (Department of Microbiology, College of medicine, Konyang University)
Jeon, Young-Ha (Department of Microbiology, College of medicine, Konyang University)
Yang, Hyo-Seon (Department of Microbiology, College of medicine, Konyang University)
Lee, Kyung-Bok (Department of Biochemistry, College of medicine, Konyang University)
Song, Kyung-Sik (Division of Applied Biology & Chemistry, Kyungpook National University)
Kang, Tae-Bong (Department of Biotechnology & Food Science, Handong University)
Kim, Jong-Bae (Department of Biotechnology & Food Science, Handong University)
Yoo, Yung-Choon (Department of Microbiology, College of medicine, Konyang University)
Publication Information
Korean Journal of Pharmacognosy / v.41, no.2, 2010 , pp. 122-129 More about this Journal
Abstract
Mistletoe (Viscum album) is a common name for many species of semi-parasitic plants which grow on deciduous trees all over the world. In this study, the immunomodulatory activity of the water-extract of Korean mistletoe fruits (KMFWE), was investigated on murine peritoneal macrophages. The culture supernatants of KMF-WE-stimulated murine peritoneal macrophages showed the increased production of IFN-$\gamma$, IL-$1{\beta}$ and TNF-$\alpha$, in a dose-dependent manner. KMF-WE also induced chemokine production from murine peritoneal macrophages such as RANTES, MCP-1, MIP-$1{\alpha}$ and MIP-$1{\beta}$, as well as nitric oxide (NO) production, in a dose-dependent manner. The gel filtration fraction revealed F-1, which is the early-eluted and high molecular weight product, is the major fraction of KMF-WE to activate the murine peritoneal macrophage to induce cytokines, chemokines and NO. The nature of F-1 fraction needs to be examined in detail in further studies to define the regulatory mechanisms of cytokine or chemokine induction by KMF-WE on macrophages. These results suggest that KMF-WE possess a potent immunostimulant activity and can be a promising candidate available for development of immunomodulators.
Keywords
Viscum album; fruit-water extract; murine peritoneal macrophage; cytokines; chemokines;
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