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Inhibitory Effects of 23 Korean Local Plant Extracts on Aldo-keto reductase family 1 B10 (AKR 1 B10)  

Lee, Joo-Young (Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
Song, Dae-Geun (Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
Jung, Sang-Hoon (Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
Kim, Jong-Hwan (Specialty Crops Experiment Station, Gangwondo Agricultural Research and Extension Services)
Ahn, Soo-Young (Specialty Crops Experiment Station, Gangwondo Agricultural Research and Extension Services)
Nho, Chu-Won (Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
Pan, Cheol-Ho (Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
Publication Information
Korean Journal of Pharmacognosy / v.40, no.3, 2009 , pp. 238-243 More about this Journal
Abstract
We examined ethanol extracts prepared from 23 Korean local plants obtained in Pyeongchang, Gangwon-do for their inhibitory effects on recombinant human AKR 1 B10 (rhAKR1B10) in vitro. To do this, rhAKR1B10 was first expressed in E. coli as a biological active form and purified by using Ni-affinity chromatography followed by gel permeation chromatography. Then, rhAKR1B10 was used for screening out 23 Korean local plant extracts having an inhibitory activity against itself. Among them, six extracts showed more than 50% inhibition of rhAKR1B10 activity at the concentration of $10{\mu}g$/ml. Especially, the extracts of Ligularia fischeri var. spiciformis Nakai and Rhus trichocarpa Miq. were the most potent because their $IC_{50}$ values were 2.94 and $2.00{\mu}g$/ml, respectively.
Keywords
Aldo-keto reductase family 1 B10; aldose reductase-like 1; anti-cancer; Ligularia fischeri; Rhus trichocarpa;
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