Browse > Article

Anti-proliferative Components in the Roots Extract from Pueraria thunbergiana  

Baek, Mok-Ryeon (Korea Research Institute of Chemical Technology)
Choi, Yeon-Hee (Korea Research Institute of Chemical Technology)
Yoo, Dae-Seok (Korea Research Institute of Chemical Technology)
Kim, Mi-Ri (Korea Research Institute of Chemical Technology)
Choi, Sang-Un (Korea Research Institute of Chemical Technology)
Hong, Kyung-Sik (Korea Research Institute of Chemical Technology)
Kim, Young-Sup (Korea Research Institute of Chemical Technology)
Kim, Young-Kyoon (College of Forest Science, Kookmin University)
Lee, Kang-Ro (College of Pharmacy, Sungkyunkwan University)
Ryu, Shi-Yong (Korea Research Institute of Chemical Technology)
Publication Information
Korean Journal of Pharmacognosy / v.40, no.1, 2009 , pp. 46-50 More about this Journal
Abstract
The antitumor activity of the roots extract of Pueraria thunbergiana was investigated on the basis of cytotoxicity upon the cultured human tumor cell lines, in vitro. The purification of methylene chloride (MC) soluble part and ethylacetate (EA) soluble part of extract by column chromatography furnished seven isoflavonoids, two triterpenoids, one but-2-enolide. The structures of them were established by chemical and spectroscopic means to be lupeol (1), $\beta$-sitosterol (2), biochanin A (3), (-)-tuberosin (4), calycosin (5), daidzein (6), puerarin (7), daidzin (8), (+)-puerol-B 2-O-$\beta$-glucopyranoside (9), formononetin-7-O-$\beta$-glucopyranoside (10). Each isolates ($1{\sim}10$) were evaluated for inhibitory activities on the proliferation of cultured human tumor cell lines such as A549, SK-OV-3, HCT-15 and SK-MEL-2, respectively.
Keywords
Pueraria thunbergiana; Leguminosae; Isoflavonoid; Cytotoxic activity;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By SCOPUS : 3
연도 인용수 순위
1 Yeung, D. K. Y., Leung, S. W. S., Xu, Y. C., Vanhoutte, P. M. and Man, R. Y. K. (2006) Puerarin, an isoflavonoid derived from Radix Pueraria, potentiates endothelium-independent relaxation via the cyclic AMP pathway in porcine coronary artery. Euro. J. Pharmacol. 552: 105–111   DOI   ScienceOn
2 Park, E. K., Shin, J., Bae, E. A., Lee, Y. C. and Kim, D. H. (2006) Intestinal bacteria activate estrogenic effect of main constituents puerarin and daidzin of Pueraria thunbergiana. Biol. Pharm. Bull. 29: 2432-2435   DOI   ScienceOn
3 Hisashi, K. N. (2003) Allelopathic substances in Pueraria thunbergiana. Phytochemistry 63: 577-580   DOI   PUBMED   ScienceOn
4 Lee, H. J., Lee, O. K., Kwon, Y. H., Choi, D. H., Kang, H. Y., Kee, H. Y., Paik, K. H. and Lee, H. J. (2006) Isoflavonone glycosides from the bark of Amorpha Fruticosa. Chemistry of Natural Compounds 42: 415-418   DOI   ScienceOn
5 Shirataki, Y., Tsuzuku, T., Yokoe, I., Hirano, R. T. and Komatsu, M. (1990) Studies on the constituents of Sophora species. XXIII. constituents of the root of Sophora chrysophylla seem. Chem. Pharm. Bull. 38: 1712-1716   DOI
6 Chang, Y. C., Nair, M. G., Santell, R. C. and Helferich, W. G. (1994) Microwave-mediated synthesis of anticarcinogenic isoflavones from soybeans. J. Agric. Food Chem. 42: 1869-1871   DOI
7 Arao, T., Kinjo, J., Nohara, T. and Isobe, R. (1997) Oleanenetype triterpene glycosides from Pueraria radix. IV. six new saponins from Pueraria Lobata. Chem. pharm. bull. 45: 362-366   PUBMED   ScienceOn
8 Skehan, P., Streng, R., Scudiero, D., Monks, A., McMahon, J., Vistica, D., Warren, J. T., Bokesch, H., Kenney, S. and Boyd, M. R. (1990) New colorimetric cytotoxicity assay for anticancer-drug screening. J. Natl. Cancer. Inst. 82: 1107-1112   DOI   PUBMED
9 Han, T., Li, H., Zhang, Q., Zheng, H. and Qin, L. (2006) New thiazinediones and other components from Xanthium Strumarium. Chemistry of Natural Compounds 42: 567-570   DOI   ScienceOn
10 Herberlt L. Holland, Peter R. P. Diakow and Gregg J. Taylor, (1978) $^{13}C$ nuclear magnetic resonance spectra of some C-19-hydroxy, C-5,6 epoxy, C-24 ethyl, and C-19-norsteroids. Can. J. Chem. 56: 3121-3127   DOI
11 Jun, M., Fu, H. Y., Hong, J., Wan, X., Yang, C. S. and Ho, C. T. (2003) Comparison of antioxidant activities of isoflavones from kudzu root (Pueraria lobata Ohwi). Journal of Food Science. 68: 2117-2122   DOI   ScienceOn
12 Park, C. H., Lim, S. S. and Lee, D. U. (2007) Structure-activity relationships of components from the roots of Pueraria thunbergiana having aldose reductase inhibitory and antioxidative activity. Bull. Korean Chem. Soc. 28: 493-495   DOI   ScienceOn
13 Reynolds, W. F. Mclean, S., Poplawski, J., Enriquez, R. G., Laura I. and Leon, I. (1986) Total assignment of $^{13}C$ and $^{1}H$ spectra of three isomeric triterpenol derivatives by 2D NMR: an investigation of the potential utility of $^{1}H$ chemical shifts in structural investigations of complex natural products. Tetrahedron 42: 3419-3428   DOI   ScienceOn
14 Du, X., Bai, Y., Liang, H., Wang, Z., Zhao, Y., Zhang, Q and Huang, L. (2006) Solvent effect in $^{1}H$ NMR spectra of 3'-hydroxy-4'-methoxy isoflavonoids from Astragalus membranaceus var. mongholicus. Magn. Reson. Chem. 44: 708-712   DOI   ScienceOn
15 Nohara, T., Kinjo, J., Furusawa, J., Sakai, Y., Inoue, M., Shirataki, Y., Ishibashi, Y., Yokoe, I. and Komatsu, M. (1993) But-2-enolides from Pueraria Lobata and revised structures of puerosides A, B and sophoroside A. Phytochemistry 33: 1207-1210   DOI   ScienceOn
16 Bae, E. A., Han, M. J. and Kim, D. H. (2001) In vitro antihelicobacter pylori activity of irisolidone isolated from the flowers and rhizomes of Pueraria thunbergiana. Planta Med. 67: 161-163   DOI   ScienceOn
17 Hirakura, K., Morita, M., Nakajima, K., Sugama, K., Takagi, K., Nitsu, K., Ikeya, Y., Maruno, M. and Okada, M. (1997) Phenolic glucosides from the root of Pueraria Lobata. Phytochemistry 46: 921-928   DOI   ScienceOn
18 Kanakubo, A., Koga, K., Isobe, M., Fushimi, T., Saitoh, T., Ohshima, Y. and Tsukamoto, Y. (2001) First finding of daidzein 7-O-phosphate and genistein 7-O-phosphate that are hydrolyzed by sulfatase. Tetrahedron 57: 8801-8805   DOI   ScienceOn
19 Kim, M. J., Lee, J. S., Ha, O. M., Jang, J. Y. and Cho, S. Y. (2002) Effects of Pueraria thunbergiana bentham water extracts on hepatic alcohol metabolic enzyme system in rats. J. Korean Soc. Food Sci. Nutr. 31: 92-97   DOI