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Poncirin alleviates the symptoms of dextran sulfate sodium-induced colitic mice  

Kim, Jong-Bin (Department of Pharmaceutical Biochemistry, College of Pharmacy)
Cho, Woong (Department of Pharmaceutical Biochemistry, College of Pharmacy)
Han, Ar-Reum (College of Pharmacy and Center for Cell Signaling & Drug Discovery Research, Ewha Womans University)
Seo, Eun-kyung (College of Pharmacy and Center for Cell Signaling & Drug Discovery Research, Ewha Womans University)
Lee, Kyung-Tae (Department of Pharmaceutical Biochemistry, College of Pharmacy)
Publication Information
Korean Journal of Pharmacognosy / v.39, no.2, 2008 , pp. 104-109 More about this Journal
Abstract
We previously reported that anti-inflammatory properties of poncirin, isolated from fruit of Poncirus trifoliata, might be the result from the inhibition of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis $factor-{\acute{a}}$ ($TNF-{\alpha}$) and interlukin-6 (IL-6) expression via the down-regulation of $NF{-\kappa}B$ binding activity. In this study, we investigated whether poncirin has an inhibitory effect on the production of pro-inflammatory mediators ex vivo and whether poncirin could relieve the symptoms of dextran sulfate sodium (DSS)-induced colitis in mice model of inflammatory bowel disease. Poncirin significantly inhibited the productions of NO, IL-6 and $TNF-{\alpha}$ in lipopolysaccharide (LPS)-induced mouse peritoneal macrophage. In addition, poncirin-treated mice when compared to control mice not receiving treatment recovered better from the weight loss caused by DSS-induced colitis. Changes in disease activity index (DAI) of poncirin-treated mice were also more favorable than for control mice and were comparable with mice treated with a typical anti-inflammatory-drug, 5-aminosalichylic acid (5-ASA). In addition, suppression of plasma NO and IL-6 productions of poncirin-treated mice was also observed in DSS-induced colitis. These results suggest that poncirin has potentially useful anti-inflammatory effects mediated by suppression of inflammatory mediator productions.
Keywords
Poncirin; Anti-inflammation; Peritoneal macrophage; DSS; Colitis;
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1 Sarto, R. B. (1997) Pathogenesis and immune mechanisms of chronic inflammatory bowel diseases, Am. J. Gastroenterol., 92: 5S-11S
2 Ramakers, J. D., Verstege, M. I., Thuijls. G., Te Velde, A. A., Mensink, R. P. and Plat J. (2007) The $PPAR-{\gamma}$ agonist rosiglitazone impairs colonic inflammation in mice with experimental colitis. J. Clin. Immunol., 27: 275-283   DOI
3 Adachi, M., Kurotani, R., Morimura, K., Shah, Y., Sanford, M., Madison, B. B., Gumucio, D. L., Marin, H. E., Peters, J. M., Young, H. A. and Gonzalez, F. J. (2006) Peroxisome proliferator activated receptor gamma in colonic epithelial cells protects against experimental inflammatory bowel disease. Gut, 55: 1104-1113   DOI   ScienceOn
4 Porter, S. N., Howarth, G. S. and Butler, R. N. (1998) An orally administered growth factor extract derived from bovine whey suppresses breath ethane in colitic rats. Scand. J. Gastroenterol., 33: 967-974   DOI
5 Fan, H., Qiu, M. Y., Mei,.J. J., Shen, G. X., Liu, S. L. and Chen, R. (2005) Effects of four regulating-intestine prescriptions on pathology and ultrastructure of colon tissue in rats with ulcerative colitis. World. J. Gastroenterol., 11: 4800-4806   DOI
6 Chang, D. K., Kim, Y. H., Byeon, J. S., Yang, S. K., Chung, Y. W., Han, D. S., Kim, S. G., Kim, T. I., Kim, W. H., Jeen, Y. T., Eun, C. S., Choi, H., Choi, K. Y. and Song, I. S. (2005) The current status of ulcerative colitis-associated colorectal cancer in Korea: a KASID study. Korean J Gastroenterol., 46: 276-282
7 Kim, H. M., Kim, H. J. and Park, S. T. (1999) Inhibition of immunoglobulin E production by Poncirus trifoliata fruit extract. J. Ethnopharmacol., 66: 283-288   DOI   ScienceOn
8 Han, A. R., Kim, J. B., Lee, J., Nam, J. W., Lee, I. S., Shim, C. K., Lee, K. T. and Seo, E. K. (2007) A new flavanone glycoside from the dried immature fruits of Poncirus trifoliata. Chem. Pharm. Bull (Tokyo)., 55: 1270-1273   DOI   ScienceOn
9 Fiocchi, C. (1998) Inflammatory bowel disease: aetiology and pathogenesis, Gastroenterology, 115: 182-205   DOI   ScienceOn
10 Kim, D. H., Bae, E. A. and Han, M. J. (1999) Anti-Helicobacter pylori activity of the metabolites of poncirin from Poncirus trifoliata by human intestinal bacteria. Biol. Pharm. Bull., 22: 422-424   DOI
11 Lee, Y. M., Kim, C. Y., Kim, Y. C. and Kim, H. M. (1997) Effects of Poncirus trifoliata on type I hypersensitivity reaction. Am. J. Chin. Med., 25: 51-56   DOI   ScienceOn
12 Eaden, J. A., Abrams, K. R. and Mayberry, J. F. (2001) The risk of colorectal cancer in ulcerative colitis: a meta-analysis. Gut, 48: 526-535   DOI
13 Kim, J. B., Han, A. R., Park, E. Y., Kim, J. Y., Cho, W., Lee, J., Seo, E. K. and Lee, K. T. (2007) Inhibition of LPSinduced iNOS, COX-2 and cytokines expression by poncirin through the NF-kappaB inactivation in RAW 264.7 macrophage cells. Biol. Pharm. Bull., 30: 2345-2351   DOI   ScienceOn
14 Liu, J. C., Chan, P., Hsu, F. L., Chen, Y. J., Hsieh, M. H., Lo, M. Y. and Lin. J. Y. (2002) The in vitro inhibitory effects of crude extracts of traditional Chinese herbs on 3-hydroxy-3-methylglutaryl-coenzyme A reductase on Vero cells. Am. J. Chinese. Med., 30: 629-636   DOI   ScienceOn
15 Hardy, R. G., Meltzer, S. J. and Jankowski, J. A. (2000) ABC of colorectal cancer. Molecular basis for risk factors. BMJ., 321: 886-889   DOI   ScienceOn
16 Ishioka, T., Kuwabara, N., Oohashi, Y. and Wakabayashi, K. (1987) Induction of colorectal tumors in rats by sulfated polysaccharides. Crit. Rev. Toxicol., 17: 215-244   DOI
17 Juoekiewicz, J. and Zdunczyk, Z. (2004) Effects of cellulose, carboxymethylcellulose and inulin fed to rats as single supplements or in combinations on their caecal parameters. Comp. Biochem. Physiol. A. Mol. Integr. Physiol., 139: 513-9   DOI   ScienceOn
18 Fiocchi, C. (2004) Inflammatory bowel disease: new insights into mechanisms of inflammation and increasingly customized approaches to diagnosis and therapy. Curr. Opin. Gastroenterol., 20: 309-310   DOI   ScienceOn
19 Park, S. H., Park, E. K. and Kim, D. H. (2005) Passive cutaneous anaphylaxis-inhibitory activity of flavanones from Citrus unshiu and Poncirus trifoliata. Planta Med., 71: 24-27   DOI   ScienceOn
20 Teng, C. M., Li, H. L., Wu, T. S., Huang, S. C. and Huang, T. F. (1992) Antiplatelet actions of some coumarin compounds isolated from plant sources. Thromb. Res., 66: 549-557   DOI   ScienceOn
21 Yuan, H. D., Jhee, O. H., Kim, S. H., Kim, H. J., Om, A. S., Lee, Y. S., Baik, S. S., Kim, J. W. and Kang, J. S. (2006) The effect of curcumin on the Dextran Sulfate Sodium-induced Colitis in the Rat. Cancer. Prevention. Res., 11: 137-143
22 Frank, K., Helmut, M., Marianne, A., Volker, G., Tina, B., Jürgen, S. and Josef, R. (2001) Clinical and histopathological features of dextran sulfate sodium induced acute and chronic colitis associated with dysplasia in rats. Int. J. Colorectal. Dis., 16: 238-246   DOI
23 Shin, T. Y., Oh, J. M., Choi, B. J., Park, W. H., Kim, C. H., Jun, C. D. and Kim, S. H. (2006) Anti-inflammatory effect of Poncirus trifoliata fruit through inhibition of NF-kappaB activation in mast cells. Toxicol. In. Vitro., 20: 1071-1076   DOI   ScienceOn
24 Chun, Y. T. and Sankawa, U. (1989) Screening of antiallergic effect in traditional medicinal drugs and active constituents of aurantii fructus imaturus. Shoyakugaku Zasshi, 43: 314-323
25 Han, D. S. 대잘질환의 실험동물모델. (2002) 대한소화기학회 세미나, 125-136
26 Podolsky, D. K. (2002) Inflammatory bowel disease. N. Eng. J. Med., 347: 417-429   DOI   ScienceOn
27 Lee, Y. M., Kim, D. K., Kim, S. H., Shin, T. Y. and Kim, H. M. (1996) Antianaphylactic activity of Poncirus trifoliata fruit extract. J. Ethnopharmacol., 54: 77-84   DOI   ScienceOn