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Phenolic Compounds from the Leaves of Nelumbo nucifera Showing DPPH Radical Scavenging Effect  

Park, Chan-Hum (Department of Oriental Medicine Resources and Research Institute of Korean Oriental Medicine, Sunchon National University)
Hur, Jong-Moon (Department of Oriental Medicine Resources and Research Institute of Korean Oriental Medicine, Sunchon National University)
Song, Kyong-Sik (Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University)
Park, Jong-Cheol (Department of Oriental Medicine Resources and Research Institute of Korean Oriental Medicine, Sunchon National University)
Publication Information
Korean Journal of Pharmacognosy / v.38, no.3, 2007 , pp. 263-269 More about this Journal
Abstract
Nelumbo nucifera (Nymphaeaceae) has been widely used in a traditional oriental medicine to treat bleeding, fever, diarrhea, hemorrhoid, sunstroke, dysentery and dizziness. The leaves of this plant were refluxed with methanol, and then fractionated with organic solvents to screen the antioxidant activity using DPPH radical. Ethyl acetate and n-butanol fractions showed good DPPH radical scavenging effects and were carried out column chromatographies to isolate nine compounds. Their chemical structures were characterized as p-hydroxybenzoic acid (1), uracil (2), luteolin (3), quercetin $3-O-{\beta}-D-glucopyranoside$ (4), $rhamnetin 3-O-{\beta}-D-glucopyranoside$ (5), $isorhamnetin 3-O-{\beta}-D-glucopyranoside$ (6), $quercetin 3-O-{\beta}-D-glucuropyranoside$ (7), $quercetin 3-O-{\beta}β-D-xylofuranosyl(1{\rightarrow}2)-{\beta}-D-galactopyranoside$ (8), and adenine (9) by comparison NMR spectral data and with those in references. Compounds 1, 2, 5 and 9 were firstly isolated from this plant. Compounds 1, 3 and 4 showed potent DPPH radical scavenging activity. Especially, compound 3, luteolin showed the higher effect than ascorbic acid used as a positive control.
Keywords
Nelumbo nucifera; flavonoid; luteolin; DPPH;
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1 Lim, S. S., Jung, Y. J., Hyun, S. K., Lee, Y. S. and Choi, J. S. (2006) Rat Lens aldose reductase inhibitory constituents of Nelumbo nucifera stamens. Phytother. Res., 20: 825-830   DOI   ScienceOn
2 Miliauskas, G., Beek, T. A., Venskutonis, P. P., Linssen, J. P. H. and Sudhölter, E. J. R. (2004) Antioxidant activity of Potentilla fruticosa. J. Sci. Food Agric., 84: 1997-1999   DOI   ScienceOn
3 Akbay, P., Basaran, A. A., Undeger, U. and Basaran, N. (2003) In vitro immunomodulatory activity of flavonoid glycosides from Urtica dioica L. Phytother. Res., 17: 34-37   DOI   ScienceOn
4 Arrigo, A. P. (1999) Gene expression and the thiol redox state. Free Radic. Biol. Med., 27: 936-944   DOI   ScienceOn
5 허준 (2004) 原本東醫寶鑑. 710. 南山堂, 서울
6 Kashiwada, Y., Aoshima, A., Ikeshiro, Y., Chen, Y. P., Furukawa, H., Itoigawa, M., Fujioka, T., Mihashi, K., Cosentino, L. M., Morris-Natschke, S. L. and Lee K. H. (2005) Anti-HIV benzylisoquinoline alkaloids and flavonoids from the leaves of Nelumbo nucifera and structure–activity correlations with related alkaloids. Bioorg. Med. Chem., 13: 443-448   DOI   ScienceOn
7 권오근, 김성환, 천병열, 박채규, 손건호 (1999) 목단피로부터 항균활성 성분의 분리. 생약학회지, 30: 340-344
8 정보섭 (1990) 도해 향약대사전(식물편). 514-517. 영림사, 서울
9 Chapple, I. L. C. (1997). Reactive oxygen species and antioxidants in inflammatory diseases. J. Clin. Periodontol., 24: 287-296   DOI   ScienceOn
10 김성빈, 노승배, 류동영, 김동욱 (2005) 고지혈증 및 동맥 경화 감응 Bio F1B 햄스터에서 연잎 (Nelumbo nucifera)의 지질저하 효과. 생약학회지, 36: 229-234   과학기술학회마을
11 Cadenas, E. and Davies, K. J. A. (2000) Mitochondrial free radical generation, oxidative stress, and aging. Free Radic. Biol. Med., 29: 222-230   DOI   ScienceOn
12 Goda, Y., Kaore Hoshino, K., Ishikawa, T., ABE, Y., Nakamura, H., Otsuka, H., Takeda, Y., Tanimurq, A. and Toyoda, M. (1999) Constituents watercress: inhibitors of histamine release from RBL-2H3 cells induced by antigen stimulation. Bio. Pharm. Bull., 22: 1319-1326   DOI   ScienceOn
13 고병섭, 전동화, 장진선, 김주호, 박선민 (2006) In vitro에 서 조릿대, 연근과 연잎이 인슐린 작용 및 분비에 미치는 영향. 한국식품과학회지, 38: 144-120   과학기술학회마을
14 Cho, E. J., Yokozawa, T., Rhyu, D. Y., Kim, S. C., Shibahara, N. and Park, J. C. (2003) Study on the inhibitory effects of Korean medicinal plants and their main compounds on the 1,1-diphenyl-2-picrylhydrazyl radical. Phytomedicine, 10: 544-551   DOI   ScienceOn
15 Loizzo, M. R., Said, A., Tundis, R., Rashed, K., Statti, G. A., Hufner, A. and Menichini, F. (2007) Inhibition of angiotensin converting enzyme (ACE) by flavonoids isolated from Ail-anthus excelsa (Roxb) (Simaroubaceae). Phytother. Res., 21:32-36   DOI   ScienceOn
16 Moriyama, H., Iizuka, T., Nagai, M. and Hoshi, K., (2003) Adenine, an inhibitor of platelet aggregation, from the leaves of Cassia alata. Biol. Pharm. Bull., 26: 1361-1364   DOI   ScienceOn
17 이양숙 (2006) 싸리 추출물의 폴리페놀 함량과 생리활성. 한국식품저장유통학회지, 13: 612-622   과학기술학회마을
18 허종문, 이종호, 최종원, 황기욱, 정신교, 김문성, 박종철 (1998) 서양고추냉이 추출물과 분리한 kaempferol 배당체들의 브로모벤젠 처리 흰쥐에서 in vitro 지질과산화억제 효과. 생약학회지, 29: 231-236
19 Moon, J. H., Tsushida, T., Nakahara, K. and Terao, J. (2001) Identification of quercetin 3-O-${\beta}$-D-glucuronide as an antioxidative metabolite in rat plasm after oral administration of quercetin. Free Radic. Biol. Med., 30: 1274-1285   DOI   ScienceOn
20 Furukawa, H. (1966) On the alkaloids of Nelumbo nucifera Gaertn. . alkaloids of Loti Embryo. Yakugaku Zasshi, 86: 75- 77   DOI
21 Lee, S. H., Kang, S. S. and Shin, K. H. (2002) Coumarins and a pyrimidine from Angelica gigas roots. Natural Product Sciences, 8: 58-61
22 Ahmed, M. S., El Tanbouly, N. D., Islam, W. T., Sleem, A. A. and El Senousy, A. S. (2005) Antiinflammatory flavonoids from Opuntia dillenii (Ker-Gawl) Haw. flowers growing in Egypt. Phytother. Res., 19: 807-808   DOI   ScienceOn
23 Donnini, S., Finetti, F., Lusini, L., Morbidelli, L., Cheynier, V., Barron, D., Williamson, S., Waltenberger, J. and Ziche, M. (2006) Divergent effects of quercetin conjugates on angiogenesis. Br. J. Nutr., 95: 1016-1023   DOI   ScienceOn
24 김창민 (1998) 중약대사전. 4623-4624. 정담, 서울
25 Ono, Y., Hattori, E., Fukaya, Y., Imai, S. and Ohizumi, Y. (2006) Anti-obesity effect of Nelumbo nucifera leaves extract in mice and rats. J. Ethnopharmacol., 106: 238-244   DOI   ScienceOn
26 Kim, S. J., Cho, J. Y., Wee, J. H., Jang, M. Y., Kim, C., Rim, Y. S., Shin, S. C., Ma, S. J., Moon, J. H. and Park, K. H. (2005) Isolation and characterization of antioxidative compounds from the aerial parts of Angelica keiskei. Food Sci. Biotechnol., 14: 58-63
27 Slater, T. F. (1984) Free radical mechanisms in tissue injury. Biochem. J. 222: 1-15   DOI
28 Halliwell B. and Gutteridge J. M. C. (1999) Free radicals in biology and medicine. 105-245. Oxford University Press, New York
29 Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T. D., Mazur, M. and Telser, J. (2007) Free radicals and antioxidants in normal physioilogical functions and human disease. Int. J. Biochem. Cell Biol., 39: 44-84   DOI   ScienceOn
30 이창복 (1993) 대한식물도감. 339-340. 향문사, 서울