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HPLC Analysis of Saponins in Platycodi Radix  

Kim, Hyun-Ki (Korea Research Institute of Chemical Technology)
Choi, Jae-Seok (Korea Research Institute of Chemical Technology)
Yoo, Dae-Seok (Korea Research Institute of Chemical Technology)
Choi, Yeon-Hee (Korea Research Institute of Chemical Technology)
Yon, Gyu-Hwan (Korea Research Institute of Chemical Technology)
Hong, Kyung-Sik (Korea Research Institute of Chemical Technology)
Lee, Byung-Hoi (Korea Research Institute of Chemical Technology)
Kim, Hye-Jin (Korea Research Institute of Chemical Technology)
Kim, Eun-Ju (JangSaeng Doraji Research Institute of Biotechnology, JangSaeng Doraji Co. LTD)
Park, Byoung-Keun (JangSaeng Doraji Research Institute of Biotechnology, JangSaeng Doraji Co. LTD)
Jeong, Young-Chul (Division of Food science, Jinju International University)
Kim, Young-Sup (Korea Research Institute of Chemical Technology)
Ryu, Shi-Yong (Korea Research Institute of Chemical Technology)
Publication Information
Korean Journal of Pharmacognosy / v.38, no.2, 2007 , pp. 192-196 More about this Journal
Abstract
A rapid and practical HPLC assay was developed for quantitative analysis of saponins in Platycodi Radix. Seven saponin components in Platycodi Radix, i.e., deapioplatycoside E, platycoside E, deapioplatycodin D$_3$, platycodin D$_3$, polygalacin D$_2$, platycodin D$_2$ and platycodin D were successfully resolved on C18 column and detected by ELSD. Standard curves were linear over the concentration range 1 ${\sim}$2,000 ${\mu}$g/ml (r$^2$>0.992). Intra- and inter-day coefficients for variation of seven saponin components were < 10% and limit of quantification of them were around 0.7${\sim}$1.5 ${\mu}$g/ml, respectively. Using this method, contents of seven saponins in various plant materials under different cultivating conditions were estimated.
Keywords
Platycodon grandiflorum; campanulaceae; saponin; HPLC; quantitative analysis;
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