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Comparison of Triterpenoid Contents of the Four Rubus Plants in Korea Using TLC-DM  

Nam, Jung-Hwan (Department of Botanical Resources, Sangji University)
Jung, Hyun-Ju (College of Pharmacy, Wonkwang University)
Choi, Jong-Won (College of Pharmacy, Kyungsung University)
Kim, Won-Bae (National Alpine Agricultural Institute, Rural Development Administration)
Park, Hee-Juhn (Department of Botanical Resources, Sangji University)
Publication Information
Korean Journal of Pharmacognosy / v.38, no.2, 2007 , pp. 187-191 More about this Journal
Abstract
The extraction yield of the methanolic extracts and 19${\alpha}$-hydroxyursane-type triterpenoid (19${\alpha}$-HUT) fraction were investigated in the unripe and ripe fruits and the leaves of the four Rubus plants (Rubus coreanus, R. crataegifolius, R. phoenicolasius, R. pungens var. oldhami) to develop the biomaterial 19${\alpha}$-HUT mixture as functional foods. Thin layer chromatogaphy-Densitometer (TLC-DM) was used to analyze the individual quantity of 19${\alpha}$-HUTs using standard compounds (euscaphic acid, tormentic acid, 23-hydroxytormentic acid, kaji-ichigoside F$_1$, rosamultin, niga-ichigoside F$_1$). The content of methanolic extract of the fruits were higher in the ripe stage than in the unripe stage whereas the content of 19${\alpha}$-HUT mixture varied with each Rubus species. The Rubus plants containing the highest amount of 19${\alpha}$-HUTs in the leaves were R. coreanus, R. phoenicolasius and R. pungens var. oldhami while only R. cratagefolius showed the highest content in the ripe fruits. The mean of total genin content of 19${\alpha}$-HUTs was 0.94 mg/g; that of the glycosides was 0.60 mg/g. The genin quantity was found in the order of 23-hydroxytormentic acid> euscaphic acid> tormentic acid; the glycoside was observed in the order of niga-ichig-oside F$_1$> kaji-ichigoside F$_1$> rosmaultin, by which the biosynthetic pathway of 23-hydroxytormentic acid and its glucoside niga-ichigoside F$_1$ via the intermediates tormentic acid and/or rosamultin was presumed. It is also suggested that the ripe fruits of R. crataegifolius will be desirable to use as functional foods rather than unripe fruits.
Keywords
Rubus; Rubus crataegifolius; 19${\alpha}$-hydroxyursane-type tritepenoid; TLC-DM;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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1 Ko, K. and Jeon, E. (2003) Ferns, fern-allies, and seed-bearing plants of Korea, Iljinsa, Seoul, pp. 294-295
2 박희준, 정현주, 남정환, 임상철, 김원배 (2006) Rubus속 식물자원에 존재하는 19$\alpha$-hydroxyursane-type triterpenoid 의 특성과 이용, 한국자원식물학회지 19: 563-572   과학기술학회마을
3 남정환, 정현주, 최종원, 박광균, 김원배, 이명선, 박희준 (2006) 개두릅에서 칼로파낙스사포닌 월별 함량변화의 추이, 생약학회지 37: 184-189   과학기술학회마을
4 Ivorra, M. D., Paya, M., Villar, A. (1988) Hypoglycemic and insulin release effects of tormentic acid: a new hypoglycemic natural product, Planta Med. 54: 282-285   DOI   ScienceOn
5 Moon, G. S. (1991) Constituents and Uses of Medicinal Herbs, Ilweolseogak, Seoul, pp. 310-311
6 Jung, H. J., Nam, J. H., Choi, J., Lee, K. T. and Park, H. J. (2004) 19-Hydroxyursane-type Triterpenoids: Antinociceptive Anti-inflammatory Principles of the Roots of Rosa rugosa. Biol. Pharm. Bull. 28: 101-104   DOI   ScienceOn
7 Wang, J. S., Qiu, Z. Y., Xia, Y. P., Li, H. J., Ren L. Y. and Zhang, L. (2006) The protective effects of total glycosides Rubus parviflolius on cerebral ischemic in rat. Zhongguo Zhong Yao Za Zhi 31: 138-141
8 Banno, N., Akihisa, T., Tokunda, H., Yasukawa, K., Higashihara, H., Ukiya, M., Watanabe, K., Kimura, Y., Hasegawa, J. and Nishino, H. (2004) Triterpene acids from the leaves of Perilla frutescens and their antiiflammatory and anti-tumorpromoting effects, Biosci. Biotechnol. Biochem. 68: 85-90   DOI   ScienceOn
9 Nam, J. H., Jung, H. J., Choi, J., Lee, K. T. and Park, H. J. (2006) The antigastropathic and anti-rheumatic effect of niga-ichigoside $F_1$ and 23-hydroxytormentic acid isolated from the unripe fruits of Rubus coreanus in a rat model, Biol. Pharm. Bull. 29: 967-970   DOI   ScienceOn
10 박희준, 남정환, 정현주, 이명선, 이경태, 정민화, 최종원 (2005) 고지방 식이로 유도된 비만 흰쥐에서 해당근에서 분리된 Euscaphic Acid 및 Tormentic Acid의 효과, 생약학회지 36: 324-331   과학기술학회마을
11 Nam, J. H., Jung, H. J., Tapondjou, L. A., Lee, K. T., Choi, J., Kim, W. B. and Park, H. J. (2007) The Anti-hyperlipidemic Effect and Constituents of the 19$\alpha$-Hydroxyursanetype Triterpenoid fraction Obtained from the Leaves of Rubus crataegifolius, Biol. Pharm. Bull. 30: (submitted)
12 Choi, J., Lee, K. T., Ha, J., Yun, S. Y., Ko, C. D., Jung, H. J. and Park H. J. (2003) Antinociceptive and anti-inflammatory effects of niga-ichigoside $F_1$ and 23-hydroxytormentic acid obtained from Rubus coreanus, Biol. Pharm. Bull. 26: 1436-1441   DOI   ScienceOn
13 Niero, R., Cechnel Filho, V., Souza, M. M., Montanari, J. L., Yunes, R. A. and Delle Monache, F. (1999) Antinociceptive activity of niga-ichigoside F1 from Rubus imperialis, J. Nat. Prod. 61: 1145-1146
14 Banno, N., Akihisa, T., Tokuda, H., Yasukawa, K., Taguchi, Y., Akazawa, H., Ukiya, M., Kimura, T. and Nishino, H. (2005) Anti-iflammatory and antitumor-promoting effects of the triterpene acids from the leaves of Eriobotrya japonica. Biol. Pharm. Bull. 28: 1995-1999   DOI   ScienceOn