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Hepatoprotective Constituents of Saururus chinensis Roots Against Tacrine-induced Cytotoxicity in Human Liver-derived Hep G2 Cells  

Jeong, Gil-Saeng (X-ray Microscopy Research Center, Wonkwang University)
Li, Bin (College of Pharmacy, Wonkwang University)
Lee, Dong-Sung (College of Pharmacy, Wonkwang University)
Kwon, Ji-Wung (College of Life Sciences and Natural Resources, Wonkwang University)
Lee, Hye-Suk (College of Pharmacy, Wonkwang University)
Kwon, Tae-Oh (College of Life Sciences and Natural Resources, Wonkwang University)
Kim, Youn-Chul (College of Pharmacy, Wonkwang University)
Publication Information
Korean Journal of Pharmacognosy / v.38, no.2, 2007 , pp. 176-180 More about this Journal
Abstract
Five liglans, sauchinone (1), di-0-methyltetrahydrofuriguaiacin B (2), manassantin A (3), manassantin B (4) and saucerneol B (5), have been isolated from the MeOH extract of Saururus chinesis roots. The evaluation for protective effect of compounds 1-5 against tacrine-induced cytotoxicity in human liver-derived Hep G2 cells was conducted. Compounds 1,2, and 5 showed significant protective effects with the EC$_{50}$ values of74.2${\pm}$0.9, 111.3${\pm}$0.8,64.3${\pm}$0.8 ${\mu}$M, respectively. Silybin, one of the well-known hepatoprotective agents, used as a positive control, and also showed protective effect with an EC$_{50}$ value of 86.2${\pm}$0.5 ${\mu}$M.
Keywords
Saururus chinesis; Saururaceae; Hepatoprotective; Hep G2 Cells; Tacrine;
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1 Viau, C. J., Curren, R. D. and Wallace, K. (1993) Cytotoxicity of tacrine and velnacrine metabolites in cultured rat, dog, and human hepatocytes. Drug Chem. Toxicol. 16: 227- 239   DOI
2 Rho, M. C., Kwon, O. E., Kim, K., Lee, S. W., Chung, M. Y., Kim, Y. H., Hayashi, M., Lee, H. S. and Kim, Y. K. (2003) Inhibitory effects of manassantin A and B isolated from the roots of Saururus chinensis on PMA-induced ICAM-1 expression. Planta Med. 69: 1147-1149   DOI   ScienceOn
3 Song, E. K., Cho, H., Kim, J. S., Kim, N. Y., An, N. H., Kim, J. A., Lee, S. H. and Kim, Y. C. (2001) Diarylheptanoids with free radical scavenging and hepatoprotective activity in vitro from Curcuma longa. Planta Med. 67: 876-877   DOI   ScienceOn
4 Galisteo, M., Rissel, M., Sergent, O., Chevanne, M., Cillard, J., Guillouzo, A. and Lagadic-Gossmann, D. (2000) Hepatotoxicity of tacrine: occurrence of membrane fluidity alterations without involvement of lipid peroxidation. J.Pharmacol. Exp. Ther. 294: 160-167
5 Jim, L. K. and Gee, J. P. (1995) Adverse effects of drugs on the liver. In Young, L. Y and Koda-Kimble, M. A. (ed.), Applied therapeutics: The clinical use of drugs, 26-1-26-17, Applied Therapeutics, Inc., Vancouver
6 Sung, S.H., Lee, E.J., Cho, J.H., Kim, H.S. and Kim, Y.C. (2000) Sauchinone, a lignan from Saururus chinensis, attenuates CCl4-induced toxicity in primary cultures of rat hepatocytes. Biol. Pharm. Bull. 23: 666-668   DOI   ScienceOn
7 Rao, K. V. and Oruganty, R. S. (1997) An improved method for the isolation of the lignan constituents of Saururus cernuus by reverse phase column chromatography. J. Liq. Chrom. & Technol. 20: 3121-3134   DOI   ScienceOn
8 Rao, K. V. and Alvarez, F. M. (1983) Manassantins A/B and saucerneol: novel biologically active lignoids from Saururus cernuus. Tetrahedron Lett. 24: 4947-4950   DOI   ScienceOn
9 Sung, S. H., Huh, M. S. and Kim, Y. C. (2001) New tetrahydrofuran- type sesquilignans of Saururus chinensis root. Chem. Pharm. Bull. 49: 1192-1194   DOI   ScienceOn
10 Lee, W. S., Lee, D. W., Baek, Y. I., An, S. J., Cho, K. H., Choi, Y. K., Kim, H. C., Park, H. Y., Bae, K. H. and Jeong, T. S. (2004) Human ACAT-1 and -2 inhibitory activities of saucerneol B, manassantin A and B isolated from Saururus chinensis. Bioorg. Med. Chem. Lett. 14: 3109-3112   DOI   ScienceOn
11 Park, H. J., Kim, R. G., Seo, B. R., Ha, J., Ahn, B. T., Bok, S. H., Lee, Y. S., Kim, H. J. and Lee, K. T. (2003) Saucernetin- 7 and saucernetin-8 isolated from Saururus chinensis inhibit the LPS-induced production of nitric oxide and prostaglandin $E_2$ in macrophage RAW 264.7 cells. Planta Med. 69: 947-950   DOI   ScienceOn
12 Ahn, B. T., Lee, S., Lee, S. B., Lee, E. S., Kim, J. G., Bok, S. H. and Jeong, T. S. (2001) Low-density lipoprotein-antioxidant constituents of Saururus chinensis. J. Nat. Prod. 64: 1562-1564   DOI   ScienceOn
13 Grant, M. H., Duthie, S. J., Gray, A. G. and Burke, M. D. (1988) Mixed function oxidase and UDP-glucuronyltransferase activities in the human Hep G2 hepatoma cell line. Biochem. Pharmacol. 37: 4111-4116   DOI   ScienceOn
14 Watkins, P. B., Zimmermann, H. J., Knapp, M. J., Gracon, S. I. and Lewis, K. W. (1994) Hepatotoxic effects of tacrine administration in patients with Alzheimer's disease. J. Am. Med. Assoc. 271: 992-998   DOI
15 정보섭, 신민교 (1998) 도해 향약(생약)대사전(식물편), 813-814. 도서출판 영림사, 서울
16 Sung, S. H. and Kim, Y. C. (2000) Hepatoprotective diastereomeric lignans from Saururus chinensis herbs. J. Nat. Prod. 63: 1019-1021   DOI   ScienceOn