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Induction of Enhancement of Anti-Tumor Immunity by Polysaccharides Fractionated from Acanthopanx Senticosus  

Yoon, Taek-Joon (Dept. of Food & Nutrition, Yuhan College)
Sung, Ji-Yeon (Food Business R&D, Boryung Co., Ltd.)
Yu, Kwang-Won (Department of Food Science & Biotechnology, Chungju National University)
Lee, Ho (Department of Food Science & Biotechnology, Kyounggi University)
Lee, Kwang-Ho (Department of Biotechnology, College of Biomedical and Health Science, Konkuk University)
Publication Information
Korean Journal of Pharmacognosy / v.38, no.2, 2007 , pp. 117-122 More about this Journal
Abstract
The specific activation of the immune system to control cancer growth in vivo has been a long-standing goal in cancer immunology. Whole tumor Iysates have been used either alone or combined with adjuvants to induce specific immune response in vivo. Here, we examined whether freezing/thawing (F/T) colon26-M3.1 tumor cell admixed with EN-3, glycoprotein purified from Acanthopanx Senticosus, could stimulate in vivo immunity by using a murine experimental tumor metastasis model produced by colon26-M3.1 carcinoma cells. Vaccination of mice with F/T treated colon26-M3.1 carcinoma cells in combination with EN-3 as an adjuvant resulted in a significant inhibition in tumor metastasis of mice against live colon26-M3.1 carcinoma challenge. In addition, the splenocytes from vaccinated mice exhibited a higher proliferating activity and secreted interferon-${\gamma}$. These results suggest that EN-3 can be applied to immunoadjuvant to enhance the antitumor immunity in vivo.
Keywords
Acanthopanax senticosus; tumor immunity; splenocytes; cytokines;
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