Browse > Article

Effects of Alginate on the Production of Type II Collagen in Chondrocytes and on the Osteoarthritic Model of White Rabbits  

Kang, Han-Saem (ECOBIO INC.)
Kim, Gwang-Yun (ECOBIO INC.)
Jung, Il (ECOBIO INC.)
Oh, Sung-Dug (ECOBIO INC.)
Kim, Chang-Hoon (ECOBIO INC.)
Shim, Bong-Sup (ECOBIO INC.)
Park, Keun-Hyung (Department of Biological & Environmental Chemistry, College of Agriculture and Life Sciences, Chonnam National University)
Oh, Suk-Jung (ECOBIO INC.)
Publication Information
Korean Journal of Pharmacognosy / v.38, no.2, 2007 , pp. 101-107 More about this Journal
Abstract
This study was carried out to examine the in vitro effects of alginate (LVA) which is low viscosity alginic acid, on collagen type II synthesis of chondrocytes and the in vivo effect, orally administered, on cartilage degradation. Rabbit articular chondrocytes were cultured in 35 mm dishes and then LVA was treated. The effects of LVA of various viscosity (86.5 cP(LVA1),45.4 cP(LVA2), 21.2 cP(LVA3) and 9.6 cP(LVA4)) and various concentration (50, 100, 200 ${\mu}$g/ml of LVA4) on chondrocytes were determined by western blotting assay for the detection of collagen type II production. In western blotting assay, collagen type II production in chondrocytes were 1.00 in control,0.95 in LVA1, 1.41 in LVA2, 1.57 in LVA3 and 1.58 in LVA4. Collagen type II production of various concentration of LVA4 were 1.00 in control, 1.24 in 50 ${\mu}$g/ml of LVA4, 1.52 in 100 ${\mu}$g/ml of LVA4 and 1.86 in 200 ${\mu}$g/ml of LVA4. Osteoarthritis (OA) was induced in 24 rabbits by unilateral anterior cruciate ligament transection (ACLT) and randomly divided into 6 groups. The experimental group was given oral administration of 0.5 ml/kg of saline(control), 12.5 mg/kg of LVA (A12.5), 25 mg/kg of LVA (A25), 50 mg/kg of LVA (A5O), 75 mg/kg of LVA (A75) and 20 mg/kg of aceclofenac (AC) for 6 weeks after ACLT. All knees were harvested at 6 weeks after surgery and cartilage degradation was evaluated. Cartilage degradation in the control group was significantly more severe than that in the A25, A5O and A75 groups but AC group had no significant changes on the macroscopic grading scale.
Keywords
Alginate; Osteoarthritis; Type II collagen;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Howell, D. S. (1984) Pathogenesis of osteoarthritis. In Moskowitz, R. W. et al (ed.), Osteoarthritis, diagnosis and management, 129-146. Saunders company, Philadelphia, WB
2 Reginster, J. Y. (2002) The prevalence and burden of osteoarthritis. Rheumatology 41: 3-6   DOI   ScienceOn
3 Niethard, F. U. (1999) Pathogenesis of osteoarthritis-approaches to specific therapy. Am J Orthop 28: 8-10
4 Cardarelli, L., Brun, P., Bernardi, D., and Abatangelo, G. (1995) The scavenger effect of hyaluronan in synovial fluid. Wound Repair Regen 376
5 Qi, W. N., Scully, S. P. (1998) Effect of type II collagen on chondrocyte response to TGF-${\beta}$1 regulation. Exp Cell Res 241: 142-150   DOI   ScienceOn
6 Dodge, G. R., Poole, A. R. (1989) Immunohistochemical detection and immunochemical analysis of type II collagen degradation in human normal, rheumatoid, and osteoarthritic articular cartilages and in explants of bovine articular cartilage cultured with interleukin 1. J Clin Invest 83: 647-661   DOI   ScienceOn
7 Moskowitz, R. W., Davis, W., Sammarco, J., Martens, M., Baker, J., Mayor, M., et al. (1973) Experimentally induced degenerative joint lesions following partial meniscectomy in the rabbit. Arthritis Rheum 16: 397-405   DOI   ScienceOn
8 Kannan, K., Ortmann, R. A., Kimpel, D. (2005) Animal models of rheumatoid arthritis and their relevance to human disease. Pathophysiology 12: 167-181   DOI   ScienceOn
9 Kobayashi, K., Amiel, M., Harwood, F. L., Healey, R. M., Sonoda, M., Moriya, H., Amiel, D. (2000) The long-term effects of hyaluronan during development of osteoarthritis following partial meniscectomy in a rabbit model. Osteoarthritis Cart 8: 359-365   DOI   ScienceOn
10 Hill, C. L., Seo, G. S., Gale, D., Totterman, S., Gale, M. E., Felson, D. T. (2005) Hyaluronan oligosaccharide-induced activation of transcription factors in bovine articular chondrocytes. Arthritis Rheum 52: 794-799   DOI   ScienceOn
11 Hollander, A. P., Pidoux, I., Reiner, A., Roraeck, C., Bourne, R., Poole, A. R. (1995) Damage to type II collagen in aging and osteoarthritis starts at the articular surface, originates around chondrocytes, and extends into the cartilage with progressive degeneration. J Clin Invest 96: 2859-2869   DOI   ScienceOn
12 Bucci, L. R. (1994). Chondroprotective agents. Glucosamine salts and chondroitin sulfates. Townsend Letter for Doctors 1: 52-54
13 Rogart, J. N., Barrach, H. J., Chichestert, C. O. (1999) Articular collagen degradation in the Hulth-Telhag model of osteoarthritis. Osteoarthritis Cart 7: 539-547   DOI   ScienceOn
14 Yoshioka, M., Coutts, R. D., Amiel, D., Hacker, S. A. (1996) Characterization of a model of osteoarthritis in the rabbit knee. Osteoarthritis and Cart 4: 87-98   DOI   ScienceOn
15 Hashimoto, S., Achermann, L. C., Takahashi, K., Amiel, D., Coutts, R. D., Lotz, M. (2002) Development and regulation of osteophyte formation during experimental osteoarthritis. Osteoarthritis Cart 10: 180-187   DOI   ScienceOn
16 Mukherjee, D., Nissen, S. E., Topol, E. J. (2001) Risk of cardiovascular events associated with selective COX-2 inhibitors. J Am Med Assoc 286: 954-959   DOI   ScienceOn
17 Solomon, D. H., Schneeweiss, S., Glynn, R. J., Kiyota, Y., Levin, R., Mogun, H., Avorn, J. (2004) Relationship between selective cyclooxygenase-2 inhibitors and acute myocardial infarction in older adults. Circulation 109: 2068-2073   DOI   ScienceOn
18 Shingleton, W. D., Hodges, D. J., Brick, P., Cawston, T. E. (1996) Collagenase: a key enzyme in collagen turnover. Biochem Cell Biol 74: 759-775   DOI   ScienceOn
19 Delafuente, J. C. (2000) Glucosamine in the treatment of osteoarthritis. Rheum Dis Clin North Am 26: 1-11   DOI
20 Wolfe, M. M., Lichtenstein, D. R., Sing, G. (1999) Gastrointestinal toxicity of nonsteroidal anti-inflammatory drugs. N Engl J Med 340: 1888-1899   DOI   ScienceOn
21 Kajimoto, O., Sakamoto, K., Takamori, Y., Kajitani, N., Imanishi, T., Matsuo, R., Kajitani, Y. (1998) Therapeutic activity of oral glucosamine hydrochloride in osteoarthritis of the knee. A placebo-controlled, double-blind, cross-over study. Japanese Journal of Clinical Nutrition 20: 41-47
22 Venter, C. S., Vorster, H. H., Van Der Nest, D. G. (1990) Composition between physiological effects of konjac-glucomannan and propionate in baboons fed 'western' diets. J Nutr 120: 1046-1053   DOI
23 Lindqvist, A. B., Bockermann, R., Johansson, A. C., Nandakumar, K. S., Johannesson, M., Holmdahl, R. (2002) Mouse models for rheumatoid arthritis. Trends Genet 18: 7-13   DOI   ScienceOn
24 Hardingham, T. E., Fosang, A. J. (1992) Proteoglycans: many forms and many functions. FASEB J 6: 861-870   DOI
25 Prockop, D. J. (1998) What holds us together? Why do some of us fall apart? What can we do about it? Matrix Biol 16: 519-528   DOI   ScienceOn
26 Brandt, K. D. (1993) Shoud nonsteroidal anti-inflammatory drugs be used to treat osteoarthritis?. Rheum Dis Clin North Am 19: 29-44
27 Masuda, K., Sah, R. L., Hejna, M. J., Thonar, E. J. (2003) A novel two-step method for the formation of tissue-engineered cartilage by mature bovine chondrocytes: The alginaterecovered- chondrocyte (ARC) method. J Orthop Res 21: 139-148   DOI   ScienceOn
28 Suzuki, T., Nakai, K., Yoshie, Y., Shirai, T., Hirano, T. (1993) Effects of sodium alginates rich in guluronic and mannuronic acids on cholesterol levels and digestive organs of high-cholesterol- fed rats. Nippon Suisan Gakkaishi 59: 545-551   DOI   ScienceOn
29 Fukumoto, T., Sperling, J. W., Sanyal, A., Fitzsimmons, J. S., Reinholz, G. G., Conover, C. A., O'Driscoll, S. W. (2003) Combined effects of insulin-like growth factor-1 and transforming growth factor-${\beta}$1 on periostealmesenchymal cells during chondrogenesis in vitro. Osteoarthritis and Cart 11: 55-64   DOI   ScienceOn
30 Towheed, T. E., Anastassiades, T. P. (1999) Glucosamine therapy for osteoarthritis. J Rheumarol 26: 2294-2297