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Effect of Ethanol Extract from Salvia miltiorrhiza on Chemoprevention and Metastasis of Breast Cancer  

Shon, Yun-Hee (Intractable Diseases Research Center, Dongguk University)
Cho, Hyun-Jung (Intractable Diseases Research Center, Dongguk University)
Kim, Mee-Kyung (Intractable Diseases Research Center, Dongguk University)
Jung, Eun-Jung (Department of Pharmacology, College of Medicine, Dongguk University)
Nam, Kyung-Soo (Department of Pharmacology, College of Medicine, Dongguk University)
Publication Information
Korean Journal of Pharmacognosy / v.38, no.1, 2007 , pp. 62-66 More about this Journal
Abstract
Ethanol extract from Salvia miltiorrhiza (SME) was tested for breast cancer chemoprevention and metastasis by measuring the activites of cytochrome P45O 1A1, aromatase, ornithine decarboxylase (ODC), and matrix metalloproteinase (MMP)-9. SME significantly inhibited cytochrome P45O 1A1-mediated ethoxyresorufin O-deethylase (EROD) activity in a dose-dependent manner in a concentration range of 100${\sim}$l,200 ${\mu}g/ml$ (p<0.01). Microsomal aromatase (estrogen synthase) activity was suppressed 54.9%${\sim}$77.5% by the SME at concentration of 600${\sim}$l,200 ${\mu}g/ml$. ODC activity induced by 12-O-tet-radecanoylphorbol-13-acetate (TPA) was significantly reduced by the SME 900 and 1,200 ${\mu}g/ml$ (p<0.05) in MCF-7 breast cancer cells. In addition, SME (900 and 1,200 ${\mu}g/ml$) markedly inhibited MMP-9 activity, a key role in cancer metastasis. Therefore, SME is worth further investigation with respect to breast cancer chemoprevention or therapy.
Keywords
Salvia miltiorrhiza; cytochrome P45O 1A1; aromatase; ornithine decarboxylase; matrix metalloproteinase-9;
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