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Inhibitory Effect of Mixture of Ethanol Extracts in Agastachis Herba and Pueraria Radix on the Proliferation and $PGE_2$ Production of HT-29 Human Colon Cancer Cell Line  

Lee, Seung-Youn (Korea Medical Science Institute)
Kim, Hee-Seok (Korea Medical Science Institute)
Kim, Jeoung-Ok (Korea Medical Science Institute)
Hwang, Sung-Wan (Korea Medical Science Institute)
Hwang, Sung-Yeoun (Korea Medical Science Institute)
Publication Information
Korean Journal of Pharmacognosy / v.37, no.4, 2006 , pp. 283-289 More about this Journal
Abstract
Ethanol extracts of the whole herb of Agastachis Herba (A) and of Pueraria Radix (P) alone and of their mixture (A+P) downregulated the cell growth, cyclooxygenase-2 (COX-2) expression, prostaglandin $E_2\;(PGE_2)$, and cGMP production. A, P, and A + P inhibited the cell growth of HT-29 colon cancer cells in a concentration- and time-dependent manner but not the growth of normal colon cell, CCD-112CoN. In addition, they markedly inhibited the productions of $PGE_2$ and cGMP as well as the mRNA expression of COX-2. These data suggest that non-toxic concentration of A, P, and A + P have a significant effect on the in vitro growth of HT-29 cells, specifically through the inhibition of the $PGE_2$ production via COX-2.
Keywords
Agastachis Herba; Pueraria radix; HT-29; COX-2; $PGE_2$;
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