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Analgesic Effect and Inhibition of Prostaglandin $E_2$ Activity and Pro-inflammatory Cytokines Production by Ethyl Alcohol Extract from New Herbal Formula  

Kwon, Soon-Yong (Department of Orthopaedics, St. Mary's Hospital, The Catholic University)
Lee, Hwa-Sung (Department of Orthopaedics, St. Mary's Hospital, The Catholic University)
Lee, Soon-Hyuck (Department of Orthopaedics, Korea University Anam Hospital)
Im, Gun-Il (Department of Orthopaedics, Dongguk University Ilsan Hospital)
Kim, Si-Na (Korea Medical Science Institute)
Kim, Hee-Seok (Korea Medical Science Institute)
Hwang, Sung-Wan (Korea Medical Science Institute)
Hwang, Sung-Yeoun (Korea Medical Science Institute)
Publication Information
Korean Journal of Pharmacognosy / v.37, no.3, 2006 , pp. 136-142 More about this Journal
Abstract
New herbal formula (NHF) is the ethanol extract mixture of Puerariae radix, Artemisia capillaries and Perilla frutescens. We have Investigated the effects on anti-inflammation by NHF and attempted acetic acid induced writhing to verify the analgesic effect. Macrophages and chondrocytes were obtained from mouse and rabbit. Inflammation was induced bγ interleukin-1, tumor necrosis $factor-{\alpha}$, $interferon-{\gamma}$, and lipopolysaccharide. NHF showed strong inhibitory efficacy against cytokine-induced proteoglycan degradation, $PGE_2$ production, NO production, and MMP-9 expression in rabbit articular chondrocyte. In the writhing test, NHF exhibited a dose-dependent inhibition of writhing. Futhermore, NHF increased the activity of SOD. NHF have anti-inflammatory and analgesic activities, and could be a good herbal medicine candidate for curing of osteoarthritis.
Keywords
Anti-inflammation; Analgesic; $PGE_2$; MMPs; osteoarthritis;
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1 Oh, M. J., Lee, K. S., Son, H. Y. and Kim, S. Y (1990) Antioxidative Components of Pueraria Root. J. Kor. Soc. Food. Sci. Tech. 22(7): 793-795   과학기술학회마을
2 Kobayashi, T., Nakata, T. and Kuzumaki, T. (2002) Effect of flavonoids on cell cycle progression in prostate cancer cells. Cancer Letters 176(1): 17-23   DOI   ScienceOn
3 Liang, Y., Huang, Y., Tsai, S., Lin-Shiau, S., Chen, C. and Lin, J. (1999) Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin andrelated flavonoids in mouse macrophages. Carcinogenesis 20(10): 1645-1652   DOI   ScienceOn
4 Lee, S. H., Seo, G S. and Sohn, D. H. (2004) Inhibition of lipopolysaccharided-induced expression of inducible nitric oxide synthase by butein in RAW 264.7 cells. Biochem. Biophys. Res. Commun. 323: 125-132   DOI   ScienceOn
5 Park, B. J., Cho, M. G, Kim, K. G, et al. (1999) Effect of natural product on the inhibition of Lipopolysacchride-induced nitric oxide synthase activity in RAQ 264.7 cell culture system. Natural Product Sciences 5(3): 113-120
6 Westacott, C. I. and Sharif, M. (1996) Cytokines in osteoarthritis: Mediators or markers of joint destruction. Semin. Arthritis. Rheum. 25: 254-272   DOI   ScienceOn
7 Moon, T. C., Chung, K. C., Son, K. H., Kim, H. P., Kang, S. S. and Chang, H. W. (1998) Screening of Cyclooxygenase-2 (COX-2) Inhibitors from Natural Products. Yakhak Hoeji 42(2): 214-219
8 Lin, R. C., Guthrie, S., Xie, C. Y. et al. (1996) Isoflavone compounds extracted from Pueraria lobata suppress alcohol preference in a pharmacogenetic rat model of alcoholism. Alcohol. Clin. Exp. Res. 20(4): 659-663   DOI
9 Badger, A. M., Cook, M. N., Swift, B. A., Newmantraa, T. M., Gowen, M. and Lark, M. (1999) Inhibition of Interleukin- 1 induced proteoglycan degradation and nitric oxide production in bovine articular cartilage/chondrocyte cultures by the natural product, Hymenialdisine. J. Pharmacol. Exp. Ther. 290: 587-593
10 Lee, C. H., Han, S. H. and Min, S. G. (1995) The effects of Puerariae radix actechins administration on liver function in carbon tetrachloride-treated rats. J. Korean. Soc. Food. Sci. Nurt. 24(5): 713-719   과학기술학회마을
11 Kim, H. K., Cheon, B. S., Kim, Y H., Kim, S. Y. and Kim, H. P. (1999) Effects of naturally occuning flavonoids on nitric oxide production in the macrophage cell line RAW 264.7 and their structure-activity relationships. Biochemical Pharmacology 58(5): 759-765   DOI   ScienceOn
12 Zeng, G.Y., Cheng, Y. S., Fan, L. L., Zhou, Y. P. and Zhang, L. Y. (1982) Pharmacologic studies on Radix puerariae: effects of puerariae flavones on coronary circulation, cardiac hemodynamics and myocardial metabolism in dogs. Chinese, Med. J. 95(2): 145-150
13 Kobayashi, T., Nakata, T. and Kuzumaki, T. (2002) Effect of flavonoids on cell cycle progression in prostate cancer cells. Cancer Letters 176(1): 17-23   DOI   ScienceOn
14 Nam, K. W, Je, K. G, Lee, J. G., Han, H. J., Kang, S. K. and Mar, W. C. (2003) Inhibition of COX-2 activity and proinflammatory cytokines (TNF$-{\alpha}$ and IL-$1{\beta}$) production by water-soluble sub-fractionated parts from Bee (Apis mellifera) Venom. Arch. Pharm. Res. 26: 383-388   DOI   ScienceOn
15 Flower, R. J. (1999) New directions in cyclooxygenase research and their implications for NSAID-gastripathy. Ita.l J. Gastroenterol. 28(4): 23-27
16 Lee, J. S., Kim, E. S. and Kim, S. W. (1999) Effects of extract of Perariae radix on lipid peroxidation in ethanol-administered rats. J. Korean. Soc. Food. Sci. Nurt. 28(4): 901-906
17 Elliott, S. and Cawston, T. (2002) The clinical potential of matrix metalloproteinase inhibitors in rheumatic disorders. Drug Aging. 18: 87-99   DOI
18 Farrell, A. J., Blake, D. R., Palmer, R. M. and Moncada, J. (1992) Increased concentrations of nitric synovial fluid and serum samples suggest increased nitric oxide synthesis in rheumatic diseases. Ann. Rheum. Dis. 51: 1219-1222   DOI   ScienceOn
19 Sandy, J. D., Brwon, H. L. G. and Lowther, D. A. (1978) Degradation of proteoglycan in acticular cartilage. Biochem. Biophys. Acta 543: 539-544
20 Huh, L. H., Kim, H. C. and Lee, S. J (1987) Studies on antiinflammatory activity and its mechanism of daidzein. J. Pharmaceut. Soc. Kor. 31(1): 164-163   과학기술학회마을