Browse > Article

Mechanism for the Vascular Relaxation Induced by Butanol Extract of Agrimonia pilosa  

Hua, Cao-Li (Professional Graduate School of Oriental Medicine)
Lee, Jun-Kyung (Professional Graduate School of Oriental Medicine)
Cho, Kuk-Hyun (Professional Graduate School of Oriental Medicine)
Kwon, Tae-Oh (Medicinal Resources Research Institute (MeRRI),College of Life Science and Natural Resources, Wonkwang University)
Kwon, Ji-Woong (Medicinal Resources Research Institute (MeRRI),College of Life Science and Natural Resources, Wonkwang University)
Kim, Jin-Sook (Korea Institute of Oriental Medicine (KIOM))
Sohn, Eun-Jin (Korea Institute of Oriental Medicine (KIOM))
Lee, Ho-Sub (Professional Graduate School of Oriental Medicine,Medicinal Resources Research Institute (MeRRI))
Kang, Dae-Gill (Professional Graduate School of Oriental Medicine, Medicinal Resources Research Institute (MeRRI))
Publication Information
Korean Journal of Pharmacognosy / v.37, no.2, 2006 , pp. 67-73 More about this Journal
Abstract
The butanol extracts of Agrimonia pilosa (BAP) induced dose-dependent vascular relaxation of phenylephrine-precontracted aorta, which was abolished by removal of functional endothelium. Pretreatment of the endothelium-intact aortic tissues with $N^G$-nitro-L-arginine methyl ester (L-NAME) and 1H-[1,2,4]-oxadiazole-[$4,3-{\alpha}$]-quinoxalin-1-one(ODQ) inhibited the relaxation induced by BAP. BAP-induced vascular relaxation was also markedly attenuated by addition of verapamiI, while the relaxant effect of BAP was not blocked by indomethacine, glibenclamide, tetraethylammonium (TEA), atropine, or propranolo. In addition, incubation of endothelium-intact aortic rings with BAP increased the vascular production of cGMP. These results suggest that BAP relaxes vascular smooth muscle via endothelium-dependent nitric oxide/cGMP signaling pathway, which may be causally related with L-type $Ca^{2+}$ channels.
Keywords
butanol extracts of Agrimonia pilosa; vasodilation; NO/cGMP;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Peach, M.J., Loeb, A.L., Singer, H.A., and Saye, J. (1985) Endothelium-derived vascular relaxaing factor. Hypertension 7: 94-100   DOI
2 Kang, D.G., Sohn, E.J., Kwon, E.K., Han, J.H., Oh, H., and Lee, H.S. (2002) Effects of berberine on angiotensinconverting enzyme and NO/cGMP system in vessels. Vascul. Pharmacol. 39: 281-286   DOI   ScienceOn
3 Lee, J.W., Roh, T.C., Rho, M.C., Kim, Y.K., and Lee, H.S. (2002) Mechanisms of relaxant action of a pyranocoumarin from Peucedanum japonicum in isolated rat thoracic aorta. Planta Med. 68: 891-895   DOI   ScienceOn
4 심민교 (1990), 임상본초학, 영림사, 서울, p. 499
5 Pei, Y.H., Li, X., and Zhu, T.R. (1990) Studies on the structure of a new ellagic acid glycoside from the rootsprouts of Agrimonia pilosa Ledeb. Yao Xue Xue Bao. 25: 798-800
6 Bradford M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254   DOI   ScienceOn
7 MacNaul, K.L. and Hutchinson, N.I. (1993) Differential of iNOS and cNOS mRNA in human vascular smooth muscle cells and endothelial cells under normal and inflammatory conditions. Biochem. Biophys. Res. Commun. 196: 1330-1334   DOI   ScienceOn
8 Kuramochi, T, Chu, J., and Suga, T. (1994) Gou-Teng (From Uncaria rhynchophylla Miquel)-induced endothelium-dependent and independent relaxations in the isolated rat aorta. Life Sci. 54: 2061-2069   DOI   ScienceOn
9 Rodriguez-Cruz, M.E., Perez-Ordaz, L., Serrato-Barajas, B. E., Juarez-Oropeza, M.A., Mascher, D., and Paredes-Carbajai, M.C. (2002) Endothelium-dependent effects of the ethanolic extract of the mistletoe Psittacanthus calyculatus on the vasomotorresponses of rat aortic rings. J. Ethnophamacol. 39: 281-286
10 Meng, W., Ma, J., Ayata, C., Hara, H., Huang, P.L., Fishman, M.C., and Moskowitz, M.A. (1996) Ach dilates pial arterioles in endothelial and neuronal NOS knockout mice by NO-dependent mechanisms. Am. J. Physiol. 271: H1145-H1150
11 Lee, T.H., Wang, G.J., Lee, C.K., Kuo, Y.H., and Chou, C.H. (2002) Inhibitory effects of glycosides from the leaves of Melaleuca quinquenervia on vascular contraction of rats. Planta Med. 68: 492-496   DOI   ScienceOn
12 Furchgott, R.F. and Zawadzki, J.V. (1980) The obligatory role of endothelial cells in therelaxation of arteterial smooth muscle by acetylcholine. Nature 288: 373-376   DOI   ScienceOn
13 Mikkelsen, E.O., Thastrup, O., and Christensen, S.B. (1998) Effects of thapsigargin in isolated rat thoracic aorta. Pharmacol. Toxicol. 62: 7-11   DOI   ScienceOn
14 Chen, Z.Y.,Zhang, Z.S., Kwan, K.Y, Zhu, M., Ho, W.K., and Huang, Y. (1998) Endothelium-dependent relaxation induced by hawthorn extract in rat mesenteric artery. Life Sci. 63: 1983-1991   DOI   ScienceOn
15 Kang, D.G., Lee, J.K. Choi, D.H. Sohn, E.J. Moon, M.K., and Lee, H.S. (2005) Vascular relaxation by the methanol extract of Sorbus cortex via NO/cGMP pathway. Biol. Pharm. Bull. 28: 860-864   DOI   ScienceOn
16 Nevala, R., Paukku, K., Korpela, R., and Vapaatalo, H. (2001) Calcium-sensitive potassium channel inhibitors antagonize genistein- and daidzein-induced arterial relaxation in vitro. Life Sci. 69: 1407-1417   DOI   ScienceOn
17 Kang, D.G., Oh, H., Cho, D.K., Kwon, E.K., Han, J.H., and Lee, H.S. (2002) Effects of bulb of Fritillaria ussuriensis MAXIM on angiotensin converting enzyme and vascular release of NO/cGMP in rats. J. Ethnopharmacol. 81: 49-55   DOI   ScienceOn
18 Nishida, S. and Satoh, H. (2003) Mechanisms for the vasodilations induced by Ginkgo biloba extract and its main constituent, bilobalide, in rat aorta. Life Sci. 72: 2659-2667   DOI   ScienceOn
19 Busse, R. and Fleming, I. (1996) Endothelial dysfunction in atherosclerosis. J. Vasco Res. 33: 181-194   DOI   ScienceOn
20 Kim, S.Z., Kim, S.H., Park, J.K., Koh, G.Y., and Cho, K.W. (1998) Presence and biological activity of c-type natriuretic peptide-dependent guanylate cyclase-coupled receptor in the penile corpus cavernosum. J. Urol. 159: 1741-1746   DOI   ScienceOn
21 Kang, D.G., Hur, T.Y., Lee, G.M., Oh, H., Kwon, T.O., Sohn, E.J., and Lee, H.S. (2002) Effects of Cudrania tricuspidata water extract on blood pressure and renal functions in NO-dependent hypertension. Life Sci. 70: 2599-2609   DOI   ScienceOn
22 Yuzurihara, M., Ikarashi, Y., Goto, K., Sakakibara, I., Hayakawa, T., and Sasaki, H. (2002) Geissoschizine methyl ether, an indole alkaloid extracted from Uncariae Ramulus et Uncus, is a poent vasorelaxant of isolated rat aorta. Eur. J. Pharmacol. 444: 183-189   DOI   ScienceOn
23 Arnold, W.P., Mitta1, C.K., Katsuki, S., and Murad, F. (1977) Nitric oxide activates guanylate cyclase and increase guanosine 3':5'-cyclic monophosphate levels in various tissue preparations. Proc. Natl. Acad. Sci. USA 74: 3203-3207
24 Ko, F.N., Huang, T.F., and Teng, C.M. (1991) Vasodilatory action mechanisms of apigenin isolated from Apium graveolens in rat thoracic aorta. Biochim. Biophys. Acta 1115: 69-748-254   DOI   ScienceOn
25 Lemos, V.S., Freitas, M.R., Muller, B., Lino, Y.D., Queiroga, C.E., and Cortes, S.F. (1999) Dioclein, a new nitric oxide- and endothelium-dependent vasodilator flavonoid. Eur. J. Pharmacol. 386: 41-46   DOI   ScienceOn