Browse > Article

In vitro Screening of Medicinal Plants with Estrogen Receptor Modulation Activity  

Lee, Chang-Min (College of Pharmacy, SungKyunKwan University)
Kang, Se-Chan (College of Pharmacy, SungKyunKwan University)
Oh, Joa-Sub (College of Pharmacy, SungKyunKwan University)
Choi, Han (College of Pharmacy, SungKyunKwan University)
Li, Xue-Mei (College of Pharmacy, SungKyunKwan University)
Lee, Jae-Hyun (College of Pharmacy, SungKyunKwan University)
Lee, Mi-Hyun (College of Pharmacy, SungKyunKwan University)
Choung, Eui-Su (College of Pharmacy, SungKyunKwan University)
Kawk, Joung-Hwan (College of Pharmacy, SungKyunKwan University)
Zee, Ok-Pyo (College of Pharmacy, SungKyunKwan University)
Publication Information
Korean Journal of Pharmacognosy / v.37, no.1, 2006 , pp. 21-27 More about this Journal
Abstract
Yeast based estrogenicity assay is the simplest and useful for the assay and the discovery of novel estrogenic substances in natural specimens, The estrogen receptor(ER) modulation activity of 50% EtOH extracts of 101 traditional medicinal herbs was assessed using a recombinant yeast assay system with both a human estrogen receptor expression plasmid and a receptor plasmid. Among them, 14 species proved to be active. Pureariae Flos (flower of Puerraria thunbergiana BENTH.) had the highest estrogenic relative potency$(7.75{\times}10^{-3})$ $(EC_{50}=9.39\;{\mu}g/ml)$. The $EC_{50}$ value of $17{\beta}-estradiol$ used as the positive control was $0.073\;{\mu}g/ml)$ (Relative Potency=1.00). There results demonstrated that some of the traditional medical herb may be useful in the therapy of estrogen replacement.
Keywords
estrogen receptor (ER); phytoestrogen; yeast-based bioassay; medicinal plants;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Brzezinski, A and Debi, A (1999) Phytoestrogens: the 'natural' selective estrogen receptor modulators? Eur. J Obstet. Gynecol. Reprod Biol. 85: 47-51   DOI   ScienceOn
2 Strauss, L., Santti, R, Saarinen, N., Streng, T., joshi, S. and Makela, S. (1998) Dietary phytoestrogens and their role in hormonally dependent disease. Toxieol. Lett. 102: 349-354   DOI   ScienceOn
3 Makela, S., Davis, V L., Tally, W C., Korkman, J., Salo, L., Vihko, R, Santti, Rand Korach, K. S. (1994) Dietary estrogens act through estrogen receptor-mediated processes and show no antiestrogenicity in cultured breast cancer cells. Environ. Health Perspect. 102: 572-578   DOI
4 Landstrom, M., Zhang, J. X, Hallmans, G, Aman, P., Bergh, A, Damber, J. E., Mazur, W., Wahala, K. and Adlercreutz, H. (1998) Inhibitory effects of soy and rye diets on the development of dunning R3327 prostate adenocarcinoma in rats. Prostate 36: 151-161   DOI   ScienceOn
5 Reel, J. R, Lanb, V and Neal, B. H. (1996) Survey and assessment ofmammalian estrogen biological assays for hazard characterization. Fund Appl. Toxicol. 34: 288-305   DOI   ScienceOn
6 Lapcik, O., Hampl, R, Hill, M., Wahala, K., Maharik, N. A and Adlercreutz, H. (1998) Radioimmunoassay of free genistein in human serum. J Steriod Biochem. Mol. Biol. 64: 261-268   DOI   ScienceOn
7 Gaido, K. W, Leonard, L. S., Lovell, S., Gould, J. C., Babai, D., Portier, C. J. and McDonnell, D. P. (1997) Evaluation of chemicals with endocrine modulation activity in a yeastbased hormone receptor gene transcription assay. Toxicol. Appl. Pharmacol. 143: 205-212   DOI   ScienceOn
8 Clemons, M. and Goss, P. (2001) Estrogen and the risk of breast cancer. N Engl. J Med 344: 276-285   DOI   ScienceOn
9 Kumagai, A., Nishino, K., Shimomura, A., Kin, T. and Yamamura, Y. (1967) Effect of glycyrrhizin on estrogen action. Endocrinol. Jpn. 14: 34-38   DOI   ScienceOn
10 Kurzer, M. S. and Xu, X (1997) Dietary phytoestrogens. Ann. Rev. Nutr. 17: 353-281   DOI   ScienceOn
11 Miller, J. H. (1972) Experimets in genetics. cold spring harbor laboratory press, cold spring harbor, New York
12 Santell, R C., Chang, Y. C., Nair, M.G and Helferich, W. G (1997) Dietary genistein exerts estrogenic effects upon the uterus, mammary gland and the hypothalamic/pituitary axis in rats. J Nutr. 127: 263-269
13 Song, Y. S., Jin, C., Jung, K. J. and Park, E. H. (2002) Estrogenic effects of ethanol and ether extracts ofpropolis. J Ethnopharmacol. 82: 89-95   DOI   ScienceOn
14 Verma, S. P., Salamone, E. and Goldin, B. (1997) Curcumin and genistein, plant natural products, show synergistic inhibitory effects on the growth of human breast cancer MCF-7 cells induced by estrogenic pesticides. Biochem. Biophys.Res. Commun. 233: 692-696   DOI   ScienceOn
15 Anderson, J. J. and Gamer, S. C. (1998) Phytoestrogen and bone. Baillierres Clin. Endocrinol. Metab. 12: 543-557   DOI   ScienceOn
16 Tikkanen, M. J., Wahala, K., Ojala, S., Vihma, V and Adlercreutz, H. (1998) Effect of soybean phytoestrogen intake on low density lipoprotein oxidation resistance. Proc. Natl. Acad Sci. USA 95: 3106-3110
17 Miksicek, R. J. (1993) Commonly occurring plant flavonoids have estrogenic activity. Mol. Pharmacol. 44: 37-43
18 Albertazzi, P. and Purdie, D. W (2002) The nature and utility of the phytoesteogens : A review of the evidence. Maturitas 42: 173-185   DOI   ScienceOn
19 Sathyamoorthy, N. and Wang, T. T. Y. (1997) Differential effects of dietary phyto-oestrogens daidzein and equol on human breast cancer MCF-7 cells. Eur. J Cancer 33: 2384-2389   DOI   ScienceOn
20 Murkies, A L., Wilcox, G, and Davis, S. R., Clinical review 92: Phytoestrogens. J Clin. Endocrinol. Metab. 83: 297-303
21 Ingram, D., Sanders, K., Kolybaba, M. and Lopez, D. (1997) Case-control study of phyto-oestrogens and breast cancer Lancet 350: 990-994   DOI   ScienceOn
22 Kuiper, G G J. M., Lemmen, J. G, Carsson, B.; Corton, J. C., Safe, S. H., van der Sasg, P. T., van der Burg., B. and Gustafsson, J.-A (1998) Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor beta. Endocrinology 139: 4252-4263   DOI   ScienceOn
23 Allan, G F, Hutchins, A and Clancy, J. (1999) An ultrahighthroughput screening assay for estrogen receptor ligands. Anal. Biochem. 275: 243-247   DOI   ScienceOn
24 Lamartiniere, C. A., Murrill, W. B., Manzolillo, P. A., Zhang, J. X., Barnes, S., Zhang, X., Wei, H. and Brown, N. M. (1998) Genistein alters the ontogeny of mammary gland development and protects against chemistry-induced mammary cancer in rats. Proc. Soc. Exp. Biol. Med. 217: 358-364
25 Anthony, M. S., Clarkson, T. B., Hughes, C. L., Morgan, T. M. and Burke, G L. (1996) Soybean isoflavones improve cardiovascular risk factors without affecting the reproductive system of peripubertal rhesus monkeys. J Nutr. 126: 4350
26 Zhang, C. Z., Wang, S. X., Zhang, Y., Chen, J. P. and Liang, X. M. (2005) In vitro estrogenic activities of Chinese medicinal plants traditionally used for the management of menopausal symptoms. J Ethnopharmacol. 98: 295-300   DOI   ScienceOn
27 Dai, Q., Shu, X-O., Jin, F., Potter, J. D., Kushi, L. H., Teas, J., Gao, Y.-T. and Zheng, W. (2001) Population-based casecontrol study of soyfood intake and breast cancer risk in Shanghai. Br. J Cancer 85: 372-378   DOI   ScienceOn
28 Simpkins, J. W, Green, P. S., Gridley, K. E., Singh, M., de Fiebre, N, C. and Rajakumar, G (1997) Role of estrogens replacement therapy in memory enhancement and the prevention ofneuronal loss associated with Alzheimer's disease. Am. J Med. 103: 19S-25S
29 Soto, A. M., Sonnennschein, C., Chung, K. L., Fernandez, M. E, Olea, N. and Serrand, FO. (1995) The E-screen assay as a tool to identify estrogens: An update on estrogenic environmental pollutants. Environ. Health Perspect. 103: 113-122   DOI
30 Lissin, L. W and Cooke, J. P. (2000) Phytoestrogens and cardiovascular health. J Am. Col. Cardiol. 35: 1403-1410   DOI   ScienceOn
31 Wuttke, W, Jarry, H., Westphalen, S., Christoffel, V and Seidiova-Wuttke, D. (2003) Phytoestrogens for hormone replacement therapy? J Steroid Biochem. Mol. Biol. 83: 133-147   DOI   ScienceOn
32 Yang, N. N., Venugopalan, M., Hardikar, S. and Glasebrook, A (1996) Identification ofan estrogen response element activated by metabolites of 17$\beta$-estradiol and raloxifene. Science 273: 1222-1225   DOI   ScienceOn