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Protective Effects of the Methanol Extracts of Acanthopanax koreanum against Oxidative Stress  

Lee, Sang-Eun (Institute of Food & Culture, R&D Center for Functional Food, Pulmuone Co. Ltd.)
Son, Dong-Wook (KT&G Central Research Institute)
Yoon, Year-Pill (Institute of Food & Culture, R&D Center for Functional Food, Pulmuone Co. Ltd.)
Lee, Sang-Yun (Institute of Food & Culture, R&D Center for Functional Food, Pulmuone Co. Ltd.)
Lee, Burm-Jong (Biohealth Products Research Center, Inje University)
Lee, Sang-Hyun (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University)
Publication Information
Korean Journal of Pharmacognosy / v.37, no.1, 2006 , pp. 16-20 More about this Journal
Abstract
The protective effects of the aqueous MeOH extracts of stem and root Acanthopanax koreanum Nakai against oxidative stress were investigated. Anti-oxidant activity of the stem extract of A. koreanum was observed in the DPPH free radical scavenging $(IC_{50}=58.7\;{\mu}g/ml)$ and the SOD test $(IC_{50}=17.52\;{\mu}g/ml)$. According to data analysis of cell survival ratio of normal fibroblasts, the skin irritation by both extracts from A. koreanum was concerned. However, in the skin primary parch test, both extracts obtained Grade I, which means that there was no skin irritation. After induction of oxidative irritation, cell survival ratio of normal fibroblasts was also monitored and it turned out that stress-inducing group with both extracts had more increased cell survival ratio. The cell extension of the stress-inducing group treated with the stem extract was most dominant in morphological study. Based on these results, the stem extract of A. koreanum showed the protective effect against oxidative stress on normal fibroblast.
Keywords
Acanthopanax koreanum; Araliaceae; fibroblast; DPPH; SOD; oxidative stress;
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Times Cited By KSCI : 4  (Citation Analysis)
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