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Screening of Thrombin Inhibitor from Medicinal and Wild Plants (II)  

Ryu, Hee-Young (Department of Food and Nutrition, Andong National University)
Kwon, Yun-Sook (Department of Food and Nutrition, Andong National University)
Kum, Eun-Joo (Department of Food and Nutrition, Andong National University)
Kwon, Chong-Suk (Department of Food and Nutrition, Andong National University)
Kwon, Gi-Seok (The School of Bioresource Sciences, Andong National University)
Kim, Kun-Woo (The School of Bioresource Sciences, Andong National Universuty)
Son, Kun-Ho (Department of Food and Nutrition, Andong National University)
Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
Publication Information
Korean Journal of Pharmacognosy / v.36, no.4, 2005 , pp. 263-272 More about this Journal
Abstract
To develop safe and new anticoagulation agents from natural resources, the inhibitory activities of 291 methanol extracts, which were prepared from different parts of 197 medicinal and wild plants, against human thrombin were evaluated. Based in anti-coagulation activity determined by thrombin time and activated partial thromboplastin time, the extract of leaf of Myrica rubra was finally selected. The extract of M. rubra showed a strong thrombin inhibitory activity (above 1,819%) at 0.5 mg/ml as a final concentration, whereas aspirin showed 337% inhibition at concentration of 1.5 mg/ml. The activity of the extract remained more than 85% and 60% by heat treatment at $100^{\circ}C$ for 30min, and acid treatment at pH 2 for 60 min, respectively. Our results suggested that the extract of Myrica rubra could be the potential source as thrombin inhibitor.
Keywords
medicinal and wild plants; Myrica rubra; thrombosis; thrombin time (TT);
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