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Inhibitory Effects of the Seed Extract of Myristica fragrans on the Proliferation of Human Tumor Cell Lines  

Lee, Jung-Won (Korea Research Institute of Chemical Technology)
Lee, Sung-Ok (Korea Research Institute of Chemical Technology)
Seo, Jee-Hee (Korea Research Institute of Chemical Technology)
Yoo, Mi-Young (Korea Research Institute of Chemical Technology)
Kwon, Jee-Woong (Korea Research Institute of Chemical Technology)
Choi, Sang-Un (Korea Research Institute of Chemical Technology)
Lee, Kang-Ro (College of Pharmacy, Sungkyunkwan University)
Kwon, Dae-Young (Korea Food Research Institute)
Kim, Young-Kyoon (College of Forest Science, Kookmin University)
Kim, Young-Sup (Korea Research Institute of Chemical Technology)
Ryu, Shi-Yong (Korea Research Institute of Chemical Technology)
Publication Information
Korean Journal of Pharmacognosy / v.36, no.3, 2005 , pp. 240-244 More about this Journal
Abstract
The methanol extract of the seed of Myristica fragrans (myristicaceae) demonstrated a potent inhibition on the proliferation of cultured human tumor cells such as A549 (non small cell lung), SK-OV-3 (ovary), SK-MEL-2(melanoma), XF498 (central nerve system) and HCT-15(colon). The MeOH extract was fractionated into three portions by serial solvent partition i,e., EtOAc soluble part, BuOH soluble part and remaining water layer. Among them, the EtOAc soluble part of the extract demonstrated a potent inhibition on the proliferation of cultured human tumor cells, Bioassay-guided fractionation of the EtOAc soluble part led to the isolation of six lignan constituents, i.e., safrole(1), machilin A (2), licarin B (3), macelignan (4), mesodihydroguaiaretic acid (5) and myristargenol A (6) as well as a large amount of myristic acid as active ingredients. Structures of the isolated active components (1-6) were established by chemical and spectroscopic means.
Keywords
Myristica frograns; Myristicaceae; anti-tumor; proliferation; lignan;
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