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Cytoprotective Effects of Natural Flavonoids on Carbon Tetrachloride-Induced Toxicity in Primary Cultures of Rat Hepatocytes  

Kim, Young-Kwan (College of Pharmacy, Kyung-Hee University)
Kim, Yang-Hee (College of Pharmacy, Kyung-Hee University)
Kim, Dong-Hyun (College of Pharmacy, Kyung-Hee University)
Lee, Kyung-Tae (College of Pharmacy, Kyung-Hee University)
Publication Information
Korean Journal of Pharmacognosy / v.36, no.3, 2005 , pp. 224-228 More about this Journal
Abstract
Protective effects of various natural flavonoids on carbon tetrachloride $(CCl_4)-induced$ hepatotoxicity were investigated in primary cultured rat hepatocytes. Some of these flavonoids decreased the ALT and LDH releases induced by $CCl_4$ in A dose-dependent manner. Neohesperidin, hesperetin, baicalin, baicalein and quercetin inhibited $CCl_4-induced$ alanine aminotransferase (ALT) release. In addition, quercetin, quercitrin, neohesperidin, baicalin, baicalein and naringin reduced $CCl_4$ induced lactate dehydrogenase (LDH) leakage. Among these flavonoids, quercitrin, quercetin, baicalin and baicalein possessed potent protective effects and were selected for the further investigation on lipid peroxidation. These four flavonoids inhibited dose dependently $CCl_4-induced$ lipid peroxidation. Especially, the protective effects of quercetin and baicalein were similar to silybin as a well-known hepatoprotective agent. These results suggest that these four flavonoids have significant cytoprotective effects and possibility of therapeutic effect on chemical-induced liver diseases.
Keywords
Flavonoid; hepatocyte; carbon tetrachloride; ALT; LDH; lipid peroxidation;
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