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Inhibitory Effects of Dehydrocostuslactone Isolated from Saussureae Radix on CDK2 Activity  

Jeon, Yong-Jin (ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd)
Lee, Hong-Sub (ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd.)
Ko, Jong-Hee (ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd.)
An, Kyung-Mi (ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd.)
Yu, Seung-Woo (ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd.)
Kang, Jae-Hoon (ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd.)
Hwang, Bang-Yeon (College of Pharmacy, Chungbuk National University)
Kim, Tae-Yong (ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd.)
Yeon, Seung-Woo (ILDONG Research Laboratories, ILDONG Pharmaceutical Co. Ltd.)
Publication Information
Korean Journal of Pharmacognosy / v.36, no.2, 2005 , pp. 97-101 More about this Journal
Abstract
Saussureae Radix, the dried root of Saussurea lappa Clarke, has traditionally used for alleviating pain in abdominal distention and tenesmus, indigestion with anorexia, dysentery, nausea, and vomiting. Here we observed that methanol extracts of Saussurea Radix inhibited CDK2 activities in vitro. This inhibitory compound was isolated and identified as dehydrocostuslactone, one of the major constituents of Saussurea Radix. It is well known that dehydrocostuslactone induces apoptotic cell death. In this study, we also showed that dehydrocosruslactone inhibited cellular Rb phosphorylation and blocked cell growth at the concentration below $12\;{\mu}g/ml$ at which apoptotic cell death was not observed. Taken together, these results indicated that dehydrocostuslactone showed its anti-proliferative effects through the inhibition of CDK2 activity as well as the induction of apoptotic cell death.
Keywords
CDK2; Saussurea Radix; dehydrocostulactone; cell cycle;
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