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Evaluation of a Schzandrin C Derivative DDB-mixed Preparation(DWP-04) on Acetaminophen Detoxification Enzyme System in the Animal Model  

Park, Hee-Juhn (Division of Botanical Resources, Sangji university)
Lee, Myeong-Seon (Division of Botanical Resources, Sangji university)
Chi, Sang-Cheol (College of Pharmacy, Sungkyunkwan University)
Lee, Kyung-Tae (College of Pharmacy, Kyung-Hee University)
Shin, Young-Ho (Dae Woo Pharmaceutical Ind. Co.)
Choi, Jong-Won (College of Pharmacy, Kyungsung University)
Publication Information
Korean Journal of Pharmacognosy / v.36, no.2, 2005 , pp. 81-87 More about this Journal
Abstract
The effects of the DWP-04 [DDB:selenium yeast:glutathione (31.1 : 6.8 : 62.1 (w/w%)] on acetaminophen detoxification enzyme system were studied in rats. Treatment with DWP-04 was prevented againt acetaminophen-induiced hepatotoxicity in rat as evidenced by the decreased formation of lipid peroxide. Effect of DWP-04 on the activities of free radical-generating enzymes, free radical scavenging enzymes and glutathione-related enzymes as well as detoxification mechanism of DWP-04 against acetaminophen-treated was investigated in rat. Activities of cytochrome p450, cytochrome b5, aminopyrine demethylase and aniline hydroxylase as free radical-generating enzymes activities were decreased by the treatment with DWP-04 against acetaminophen treated. Although acetaminophen-induced hepatotoxicity results in the significantly decrease in the level of hepatic glutathione and activities of glutathine S-transferase, quinone reductase, glutathione reductase and ${\gamma}-glutamyl-$cysteine synthetase, these decreasing effects were markedly lowered in the DWP-04-treated rat. Therefore, it was concluded that the mechanism for the observed preventive effect of DWP-04 against the acetaminophen-induced hepatotoxicity was associated with the decreased activities in the free radical-generating enzyme system.
Keywords
DWP-04; DDB; selenium yeast; glutathione; acetaminophen; hepatotoxicity; microsomal enzyme; glutathione S-transferase;
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