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Effect of Radish on HeLa Cell Vacuolation Induced by Helicobacter pylori Cytotoxin  

Shon, Yun-Hee (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Surh, Jung-Ill (Department of Internal Medicine, College of Medicine, Dongguk University)
Chung, Yu-Jin (Department of Internal Medicine, College of Medicine, Dongguk University)
Park, In-Kyung (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Kim, Ho-Chang (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Hwang, Cheorl-Weon (Department of Environmental Microbiology, Handong University)
Kim, Cheorl-Ho (Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University)
Nam, Kyung-Soo (Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University)
Publication Information
Korean Journal of Pharmacognosy / v.35, no.3, 2004 , pp. 250-254 More about this Journal
Abstract
Helicobacter pylori (H. pyroli) infection it associated with type B gastritis, peptic uler disease, and gastric cancer. The vacuolation of cells induced by H. pylori is thought to be essential for the initiation and maintenance of gastric infection. The roles of H. pylori cytotoxin, urease, and ammonia in the vacuolation of HeLa cells were determined. H. pylori toxin induced vacuolation of HeLa cells. Korean and Japanese radishes significantly prevented the vacuolation of HeLa cells induced by H. pylori toxin. The urease activity in vacuolated cells was also decreased with Korean and Japanese radishes. H. Pylori toxin-induced vacuolation was inhibited by vacuolar type ATPase inhibitors (bafilomycin and N-ethylmaleimide). However, further investigation is required to determine the mechanisms of radish for the inhibition of vacuole formation of eukaryotic cells in response to the H. pylori toxin.
Keywords
Radish (Raphanus sativus L.); Helicobacter pylori; vacuolation; urease; ammonia;
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