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Effect of the Hesperetin and Naringenin on $pp60^{v-src}$-induced $NF-{\kappa}B$ Activation  

Kwon, O-Song (Laboratory of Cellular Signaling Modulators, Korea Research Institute of Bioscience and Biotechnology)
Kim, Bo-Yeon (Laboratory of Cellular Signaling Modulators, Korea Research Institute of Bioscience and Biotechnology)
Kim, Kyoung-A (Laboratory of Cellular Signaling Modulators, Korea Research Institute of Bioscience and Biotechnology)
Kim, Min-Soo (Laboratory of Cellular Signaling Modulators, Korea Research Institute of Bioscience and Biotechnology)
Oh, Hyun-Cheol (Laboratory of Cellular Signaling Modulators, Korea Research Institute of Bioscience and Biotechnology)
Kim, Beom-Seok (Laboratory of Cellular Signaling Modulators, Korea Research Institute of Bioscience and Biotechnology)
Kim, Young-Ho (Department of Manufacturing Pharmacy, Chungnam National University)
Ahn, Jong-Seog (Laboratory of Cellular Signaling Modulators, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Korean Journal of Pharmacognosy / v.35, no.3, 2004 , pp. 244-249 More about this Journal
Abstract
The effects of hesperetin and naringenin on $NF-{\kappa}B$ activation were investigated in normal rat kidney cells transformed by temperature sensitive Rous Sarcoma Virus (tsNRK). The flavonoids, naringenin and hesperetin, significantly reduced v-Src-induced $NF-{\kappa}B$ activation as well as phosphorylation of Akt and GSK-3 in tsNRK cells, whereas these compounds did not effect on platelet-derived growth factor (PDGF)-induced $NF-{\kappa}B$ activation in $NIH3T3{\gamma}l$ cells. In addition, the DNA binding activity of SP-I was also reduced but that of AP-1 was not affected by the compounds. Our study suggests that Src-induced $NF-{\kappa}B$ activation could occur via Akt-GSK-3 pathway without $IkB{\alpha}$ degradation and that naringenin and hesperetin could be used in the treatment of cancer through the inhibition of $NF-{\kappa}B$ activation.
Keywords
Naringenin; Hesperetin; Flavonoid; $NF-{\kappa}B$; Akt; GSK-3; v-Src; $I{\kappa}B{\alpha}$;
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