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Screening of Thrombin Inhibitors from Medicinal and Wild Plants  

Kwon, Yun-Sook (Department of Food and Nutrition, Andong National University)
Kim, Young-Sook (Department of Food and Nutrition, Andong National University)
Kwon, Ha-Young (Department of Food and Nutrition, Andong National University)
Kwon, Gi-Seok (The School of Bioresource sciences, Andong National University)
Kim, Kyung-Jae (Research Center for Natural & New materials, Daepyung Co.)
Kwon, Chong-Suk (Department of Food and Nutrition, Andong National University)
Son, Kun-Ho (Department of Food and Nutrition, Andong National University)
Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
Publication Information
Korean Journal of Pharmacognosy / v.35, no.1, 2004 , pp. 52-61 More about this Journal
Abstract
Inhibitory activities of 264 methanol extracts, which were prepared from different parts of 210 kinds of wild and medicinal plants, against human thrombin were evaluated. Based on the anti-coagulation activity determined by thrombin time and activated partial thromboplastin time, the 14 extracts were screened. The fibrinolytic activity, heat stability and inhibition of other proteolytic digestive enzymes, such as pepsin, papain, trypsin and chymotrypsin, of the 14 extracts were further determined, and Ginko biloba (herba), Ephedra sinica (radix), Reynoutria elliptica (herba), Amomum tsao-ko Crevost (fructus), and Magnolia officinalis Rehd. et Wils (bark) were finally selected as possible plant sources for anti-thrombosis agent. These results suggested that medicinal and wild plants could be the potential source of thrombin inhibitor.
Keywords
Thrombosis; thrombin time (TT); activated Partial Thromboplastin Time (aPTT); medicinal and wild plants;
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  • Reference
1 Korea National Statistical Office. (2000). A study on causesof death for 1999
2 Cattaneo, F., Trento, F., Pescador, R., Porta, R., and Furro, L.(2002). Pharmacodynamics of the anticoagulant activity(APTT) of an algal polysaccharide. Thrombosis Research,105: 455-457   DOI   ScienceOn
3 Gwak, H.-S. and Chun, I. K. (2000). Performulation study ofaspalatone, a new antithrombotic agent. The J. Appl.Pharmacol. 8: 332-337
4 Molina, V., Arruzazabala, M. L., and Mas, C. R. (2003).Synergistic effect of D-003 and aspirain on experimentalthrombosis models. Prostag. Leukotr. Ess. 68: 305-310   DOI   ScienceOn
5 Paik, Y.-S., Song, J.-K., Yoon, C. H., Chung, K. S., and Yun-Choi, H. S. (1995). Anti-platelet and anti-thrombotic effectsof Gastrodia elata. Kor. J. Pharmacogn. 26: 385-389
6 Yun, Y.-R, Kang, W.-S., and Lee, M.-Y. (1996). Theantithrombotic effects of green tea catechins. J. Fd Hyg.Safety, 11: 77-82
7 Yi, D.-H. and Seu, J.-H. (1982). Trypsin inhibitor fromStreptomyces sp. (Part 1) Isolation of microorganism andpurification of the inhibitor. Kor. J. Appl. Microbiol. Bioeng.10: 275-281
8 Butenas, S. and Mann, K. G. (2002). Blood coagulation.Biochemisrty (Moscow), 67: 3-12
9 Astrup, T. and Mullertz, S. (1952). The fibrin plate methodfor estimating fibrinolytic activity. Archs. Biochem. Biophys.40: 346-350   DOI   ScienceOn
10 Lee, H. J., Kim, J. S., Heo, G.-Y, Lee, K.-B., Rhee, I.-K., andSong, K.-S. (1999). Inhibitory activities of Basidiomyceteson prolyl endopeptidase, acetylcholine esterase and coagula-tion. J. Korean Soc. Aeri. Chem. Biotechnol. 42: 336-343
11 Lee, S.-S., Kim, S.-M., Park U.-Y., Kim, H.-Y., and Shin, I.-S. (2002). Studies on proteolytic and fibrinolytic activity ofBacillus subtilis JM-3 isolated from anchovy sauce. KoreanJ. Food Sci. Technol. 34: 283-289
12 Hsieh, K.-H. (1997). Thrombin interaction with fibrinpolymerization sites, Thrombosis Research, 86: 301-316   DOI   PUBMED   ScienceOn
13 Lee, T. B. (1993). Illustrated flora of Korea, Hwang MoonSa, Seoul
14 Yun-Choi, H. S., Chung, K. S., Kim, M. H., and Oh, J. H.(1995). Antithrombotic effects of some traditional plantmedicines. Kor. J. Pharmacogn. 26: 154-158
15 Hahn, B. S., Wu, S., Kim, S.-W., and Kim, Y. S. (1999).Evaluation of anticoagulant and fibrinolytic activities fromcrude extracts of insects. Kor. J. Pharmacogn. 30: 409-412
16 Weitz, J. I. and Crowther, M. (2002). Direct thrombininhibitors. Thrombosis Research, 106: 275-284   DOI   ScienceOn