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Inhibitory Effects of the Stem Bark Extract of Eucommia ulmoides on the Proliferation of Human Tumor Cell Lines  

Choi, Yeon-Hee (Korea Research Institute of Chemical Technology)
Seo, Jee-Hee (Korea Research Institute of Chemical Technology)
Kim, Jung-Sook (Korea Research Institute of Chemical Technology)
Heor, Jung-Hee (Korea Research Institute of Chemical Technology)
Kim, Seong-Kie (Korea Research Institute of Chemical Technology)
Choi, Sang-Un (Korea Research Institute of Chemical Technology)
Kim, Young-Sup (Korea Research Institute of Chemical Technology)
Kim, Young-Kyoon (College of Forest Science, Kookmin University)
Ryu, Shi-Yong (Korea Research Institute of Chemical Technology)
Publication Information
Korean Journal of Pharmacognosy / v.34, no.4, 2003 , pp. 308-313 More about this Journal
Abstract
A bioassay-guided fractionation of the stem bark extract of Eucommia ulmoides Oliver (Eucommiaceae) led to the isolation of three iridoid constituents, genipin (1), geniposide (3), geniposidic acid (4) as well as $({\pm})-guaiacylglycerol$ (2) and fatty acid mixtures as active ingredients of the extract responsible for the antitumoral property. The EtOAc soluble part and BuOH soluble part of the extract demonstrated a potent inhibition on the proliferation of cultured human tumor cells such as A549 (non small cell lung), SK-OV-3 (ovary), SK-MEL-2 (melanoma), XF498 (central nerve system) and HCT-15 (colon) in vitro, whereas the remaining water soluble part exhibited a poor inhibition. The intensive investigation of the EtOAc soluble part and BuOH soluble part of the extract yielded genipin, guaiacylglycerol, geniposide, geniposidic acid and large amounts of fatty acid mixtures as active components.
Keywords
Genipin; geniposide; geniposidic acid; Eucommia ulmoides;
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1 Li Y, Sato T, Metori K, Koike K, Che Q. M., and Takahashi S. (1998) The promoting effects of geniposidic acid and aucubin in Eucommia ulmoides oliver leaves on collagen synthesis. Biol. Pharm. Bull. 21: 1306-1310   DOI   ScienceOn
2 Nakamura T., Nakazawa Y., Onizuka S., Satoh S., Chiba A., Sekihashi K., Miura A., Yasugahira N., and Sasaki YF. (1997) Antimutagenicity of Tochu tea (an aqueous extract of Eucommia ulmoides leaves): 1. The clastogen-suppressing effects of Tochu tea in CHO cells and mice. Mutat. Res. 388:7-20   DOI   ScienceOn
3 Morota T., Sasaki H., Nishimura H., Sugama K., Chin M., and Mitsuhashi H. (1989) Two iridoid glycosides from Rhemannia glutinosa. Phytochemistry 28: 2149-2153   DOI   ScienceOn
4 DeyamaT., Ikawa T., Kitagawa S., and Nishibe S. (1987) The constituents of Eucommia ulmoides oliv, Chem. Pharm. Bull. 35: 1785-1789   DOI
5 Skehan P., Streng R., Scudiero D., Monks A., McMahon J., Vistica D., et al.(1990) New colorimetric cytotoxicity assay for anticancer-drug screening. J. Natl. Cancer Inst. 82: 1107-1112   DOI
6 Jensen S. R., Olsen C. E., Rahn K., and Rasmussen H. (1996) Iridoid glucosides in Plantago alpina and P. altissima. Phytochemistry 42: 1633-1636   DOI   ScienceOn
7 Watanabe J., Kawabata J., Kurihara H., and Niki R. (1997) Isolation and identification of alpha-glucosidase inhibitors from tochu-cha (Eucommia ulmoides). Biosci Biotechnol Biochem. 61: 177-178   DOI   ScienceOn
8 Nano J. L., Nobili C, Girard-Pipau F., and Rampal P. (2003) Effects of fatty acids on the growth of Caco-2 cells. Prostaglandins Leukot Essent Fatty Acids. 69: 207-215   DOI   ScienceOn
9 Hsu H. Y., Yang J. J., Lin S. Y., and Lin C. C. (1997) Comparisons of geniposidic acid and geniposide on antitumor and radioprotection after sublethal irradiation. Cancer Lett. 113: 31-37   DOI   PUBMED   ScienceOn
10 Sasaki Y.F., Chiba A., Murakami M., Sekihashi K., Tanaka M., Takahoko M., Moribayashi S., Kudou C., Hara Y., Nakazawa Y., Nakamura T., and Onizuka S. (1996) Antimutagenicity of Tochu tea (an aqueous extract of Eucommia ulmoides leaves): 2. Suppressing effect of Tochu tea on the urine mutagenicity after ingestion of raw fish and cooked beef. Mutat. Res. 371: 203-214   DOI   PUBMED   ScienceOn
11 Yen G. C. and Hsieh C. L. (2000) Reactive oxygen species scavenging activity of Du-zhong (Eucommia ulmoides oliv.) and its active compounds. J. Agric. Food Chem. 48: 3431-3436   DOI   ScienceOn
12 Hsieh C. L. and Yen G. C. (2000) Antioxidant actions of duzhong(Eucommia ulmoides oliv.) toward oxidative damage in biomolecules. Life Sci. 66: 1387-1400   DOI   ScienceOn
13 Harada H., Yamashita U., Kurihara H., Fukushi E., Kawabata J., and Kamei Y. (2002) Antitumor activity of palmitic acid found as a selective cytotoxic substance in a marine red alga. Anticancer Res. 22: 2587-2590
14 Oshima Y., Takata S., Hikino H., DeyamaT., and Kinoshita G. (1988) Anticomplementary activity of the constituents of Eucommia ulmoides bark. J. Ethnopharmacol. 23: 159-164   DOI   ScienceOn
15 Chang Y. C., Tseng T. H., Lee M. J., Hsu J. D., and Wang C. J. (2002) Induction of apoptosis by penta-acetyl geniposide in rat C6 glioma cells. Chem. Biol. Interact. 141: 243-257   DOI   ScienceOn
16 Lee M. J., Hsu J. D., and Wang C. J. (1995) Inhibition of 12O-tetradecanoyl-phorbol-13-acetate caused tumor promotion in benzo[a]pyrene-initiated CD-1 mouse skin by geniposide.Anticancer Res. 15: 411-416   DOI   ScienceOn